PMID- 6237550
OWN - NLM
STAT- MEDLINE
DCOM- 19841121
LR  - 20131121
IS  - 0001-6772 (Print)
IS  - 0001-6772 (Linking)
VI  - 120
IP  - 4
DP  - 1984 Apr
TI  - Enzyme levels in pools of microdissected human muscle fibres of identified type. 
      Adaptive response to exercise.
PG  - 505-15
AB  - Enzyme activities were determined in pools of type I (slow twitch) and II A and 
      II B (fast twitch) fibres of the thigh muscle from individuals engaged to a high 
      degree in physical training of an endurance character and from 
      non-endurance-trained controls. The endurance-trained (ET) group had 
      significantly higher activity levels of the mitochondrial enzymes citrate 
      synthase, malate dehydrogenase, and 3-OH-acylCoA dehydrogenase both in type I 
      (2.1X, 1.7X, 1.4X) and in type II A (2.3X, 1.8X, 1.4X) and II B fibres (2.0X, 
      1.5X, 1.5X) than the non-endurance-trained (NET) group. Of the glycolytic 
      enzymes, phosphofructokinase (PFK) in type I fibres was significantly higher 
      (1.8X) in the ET than in the NET group whereas glyceraldehydephosphate 
      dehydrogenase (GAPDH) in type I fibres was similar in the two groups. In type II 
      fibres both PFK and GAPDH levels tended to be higher in the ET group. Lactate 
      dehydrogenase (LDH) of both fibre types were not different in the two groups. 
      Type I fibres differed significantly from type II fibres for all the six enzymes 
      measured in both groups. However, no significant difference between fibres of 
      types II A and II B was found. The results indicate that fibres of types I, II A 
      and II B in human skeletal muscle all possess great adaptability with regard to 
      their oxidative capacity. Furthermore, the data suggest that extensive endurance 
      training may enhance the glycolytic capacity in both type I and type II fibres 
      although the glycolytic capacity of the muscle as a whole generally is low in 
      endurance trained subjects owing to a predominance of type I fibres. It is 
      concluded that further studies are needed to determine whether there is a 
      metabolic distinction between fibres of types II A and II B.
FAU - Essen-Gustavsson, B
AU  - Essen-Gustavsson B
FAU - Henriksson, J
AU  - Henriksson J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Acta Physiol Scand
JT  - Acta physiologica Scandinavica
JID - 0370362
RN  - EC 1.1.1.- (3-Hydroxyacyl CoA Dehydrogenases)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 1.1.1.37 (Malate Dehydrogenase)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
RN  - EC 2.3.3.1 (Citrate (si)-Synthase)
RN  - EC 2.7.1.11 (Phosphofructokinase-1)
SB  - IM
MH  - 3-Hydroxyacyl CoA Dehydrogenases/metabolism
MH  - *Adaptation, Physiological
MH  - Adult
MH  - Citrate (si)-Synthase/metabolism
MH  - Citric Acid Cycle
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/metabolism
MH  - Glycolysis
MH  - Histocytochemistry
MH  - Humans
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Malate Dehydrogenase/metabolism
MH  - Male
MH  - Mitochondria, Muscle/enzymology
MH  - Muscles/*enzymology
MH  - Oxidation-Reduction
MH  - Phosphofructokinase-1/metabolism
MH  - Physical Endurance
MH  - *Physical Exertion
EDAT- 1984/04/01 00:00
MHDA- 1984/04/01 00:01
CRDT- 1984/04/01 00:00
PHST- 1984/04/01 00:00 [pubmed]
PHST- 1984/04/01 00:01 [medline]
PHST- 1984/04/01 00:00 [entrez]
AID - 10.1111/j.1748-1716.1984.tb07414.x [doi]
PST - ppublish
SO  - Acta Physiol Scand. 1984 Apr;120(4):505-15. doi: 
      10.1111/j.1748-1716.1984.tb07414.x.