PMID- 6237684
OWN - NLM
STAT- MEDLINE
DCOM- 19841212
LR  - 20190613
IS  - 0006-2960 (Print)
IS  - 0006-2960 (Linking)
VI  - 23
IP  - 18
DP  - 1984 Aug 28
TI  - Interconversion of high and low adenosinetriphosphatase activity forms of 
      Escherichia coli F1 by the detergent lauryldimethylamine oxide.
PG  - 4140-3
AB  - The amphipathic detergent lauryldimethylamine oxide (LDAO) stimulated ATP 
      hydrolytic activity of Escherichia coli membranes and isolated ECF1 and ECF1-F0 
      ATPase complexes in a concentration-dependent manner. The enzyme was maximally 
      activated 3-fold in membranes and 5-6-fold for isolated ECF1 or the ECF1-F0 
      complex. The maximal specific activity of activated ECF1 was 140-160 mumol of ATP 
      hydrolyzed min-1 mg-1. The activation by LDAO was reversible. LDAO specifically 
      released subunit delta from ECF1, generating a four subunit enzyme (alpha, beta, 
      gamma, and epsilon subunits). The removal of subunit delta was not responsible 
      for the stimulation of ATPase activity as evidenced by the full activation of the 
      four subunit enzyme by LDAO. Treatment of ECF1 with 
      1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide generated a beta-epsilon 
      cross-link in high yield [Lotscher, H.R., DeJong, C., & Capaldi, R. A. (1984) 
      Biochemistry (accompanying paper in this issue)]. The formation of this 
      cross-link was greatly reduced in the presence of LDAO, indicating that the 
      detergent perturbated the interaction between epsilon and beta subunits although 
      epsilon was not removed from the ECF1 complex. The results suggest that the 
      interconversion of ECF1 from a low to a high ATPase activity form by LDAO is in 
      major part due to a release of the inhibitory action of subunit epsilon on 
      subunit beta.
FAU - Lotscher, H R
AU  - Lotscher HR
FAU - deJong, C
AU  - deJong C
FAU - Capaldi, R A
AU  - Capaldi RA
LA  - eng
GR  - HL 24526/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (Detergents)
RN  - 0 (Dimethylamines)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Surface-Active Agents)
RN  - 4F6FC4MI8W (dodecyldimethylamine oxide)
RN  - EC 3.6.3.14 (Proton-Translocating ATPases)
SB  - IM
MH  - Detergents/*pharmacology
MH  - Dimethylamines/*pharmacology
MH  - Escherichia coli/*enzymology
MH  - Kinetics
MH  - Macromolecular Substances
MH  - Proton-Translocating ATPases/*metabolism
MH  - Surface-Active Agents/*pharmacology
EDAT- 1984/08/28 00:00
MHDA- 1984/08/28 00:01
CRDT- 1984/08/28 00:00
PHST- 1984/08/28 00:00 [pubmed]
PHST- 1984/08/28 00:01 [medline]
PHST- 1984/08/28 00:00 [entrez]
AID - 10.1021/bi00313a020 [doi]
PST - ppublish
SO  - Biochemistry. 1984 Aug 28;23(18):4140-3. doi: 10.1021/bi00313a020.