PMID- 6238966
OWN - NLM
STAT- MEDLINE
DCOM- 19850110
LR  - 20210210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 259
IP  - 23
DP  - 1984 Dec 10
TI  - Phase separation of the receptor for immunoglobulin E and its subunits in Triton 
      X-114.
PG  - 14922-7
AB  - Above its critical micelle concentration, Triton X-114 in solution forms two 
      phases at room temperature: a lower phase containing supramicellar aggregates and 
      an upper phase largely depleted of detergent. This property of the detergent is 
      potentially useful for separating under mild conditions proteins that bind 
      detergent from those that do not (Bordier, C. (1981) J. Biol. Chem. 256, 
      1604-1607). We studied the distribution of the receptor for immunoglobulin E 
      (IgE) and its subunits in the two phases. IgE and IgE complexed either with 
      intact receptors or with the alpha chains of the receptor alone are principally 
      partitioned into the upper phase, whereas the unliganded receptor as well as the 
      isolated alpha, and especially the beta and gamma chains of the receptor, 
      preferentially partition into the lower detergent phase. Chromatography of IgE 
      and of the subunits of the receptor on a hydrophobic support showed that the beta 
      and gamma chains have a considerably greater hydrophobic surface than the alpha 
      chains or IgE. These results indicate that the distribution of a protein in the 
      two phases of phase-separated Triton X-114 is not an all-or-none effect based 
      upon whether it binds detergent or not. Rather, it reflects the overall balance 
      between the hydrophobic and hydrophilic properties of the protein's surface.
FAU - Alcaraz, G
AU  - Alcaraz G
FAU - Kinet, J P
AU  - Kinet JP
FAU - Kumar, N
AU  - Kumar N
FAU - Wank, S A
AU  - Wank SA
FAU - Metzger, H
AU  - Metzger H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Detergents)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Receptors, IgE)
RN  - 0 (Receptors, Immunologic)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - 9002-93-1 (Octoxynol)
RN  - 9036-19-5 (Nonidet P-40)
SB  - IM
MH  - Animals
MH  - Basophils/immunology
MH  - Detergents
MH  - Immunoglobulin E/isolation & purification
MH  - Leukemia, Experimental/*immunology
MH  - Macromolecular Substances
MH  - Molecular Weight
MH  - Octoxynol
MH  - Polyethylene Glycols
MH  - Rats
MH  - Receptors, IgE
MH  - Receptors, Immunologic/*isolation & purification
MH  - Temperature
EDAT- 1984/12/10 00:00
MHDA- 1984/12/10 00:01
CRDT- 1984/12/10 00:00
PHST- 1984/12/10 00:00 [pubmed]
PHST- 1984/12/10 00:01 [medline]
PHST- 1984/12/10 00:00 [entrez]
AID - S0021-9258(17)42692-5 [pii]
PST - ppublish
SO  - J Biol Chem. 1984 Dec 10;259(23):14922-7.