PMID- 624844 OWN - NLM STAT- MEDLINE DCOM- 19780417 LR - 20190723 IS - 0022-1759 (Print) IS - 0022-1759 (Linking) VI - 19 IP - 2-3 DP - 1978 TI - An immunoglobulin E assay using radiolabelled Fab' and ammonium sulfate. PG - 235-41 AB - An immunochemical assay is described in which a radiolabelled antibody fragment, Fab', is bound specifically to immunoglobulin E (IgE), and precipitated with ammonium sulfate. The radioactivity in the precipitate is a measure of the amount of IgE in the sample. Results for six serum samples are compared using the double antibody and ammonium sulfate methods as well as the PRIST. FAU - Wilcsek, R J AU - Wilcsek RJ FAU - Hamburger, R N AU - Hamburger RN LA - eng PT - Journal Article PT - Research Support, U.S. Gov't, P.H.S. PL - Netherlands TA - J Immunol Methods JT - Journal of immunological methods JID - 1305440 RN - 0 (Iodine Radioisotopes) RN - 37341-29-0 (Immunoglobulin E) RN - SU46BAM238 (Ammonium Sulfate) SB - IM MH - Ammonium Sulfate/*pharmacology MH - Animals MH - Antibody Specificity MH - Humans MH - *Immunoglobulin E MH - Iodine Radioisotopes MH - *Isotope Labeling MH - Protein Binding MH - Rabbits EDAT- 1978/01/01 00:00 MHDA- 1978/01/01 00:01 CRDT- 1978/01/01 00:00 PHST- 1978/01/01 00:00 [pubmed] PHST- 1978/01/01 00:01 [medline] PHST- 1978/01/01 00:00 [entrez] AID - 0022-1759(78)90183-7 [pii] AID - 10.1016/0022-1759(78)90183-7 [doi] PST - ppublish SO - J Immunol Methods. 1978;19(2-3):235-41. doi: 10.1016/0022-1759(78)90183-7.