PMID- 6252289
OWN - NLM
STAT- MEDLINE
DCOM- 19801216
LR  - 20031114
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 49
IP  - 1
DP  - 1980 Jul
TI  - Morphogenesis of the assembly and release of bovine enterovirus.
PG  - 225-30
AB  - Fluorescent antibody (FA) studies of cells infected with bovine enterovirus 
      showed cytoplasmic blebs with specific fluorescence to the virus. These 
      structures were also found extracellularly in the debris of lysed cells and were 
      RNA-positive by acridine orange (AO) staining. The morphology of virus-infected 
      cells was further studied by scanning electron microscopy (SEM). Transmission 
      electron microscopy (TEM) with immunoferritin tagging showed the development of 
      long sacs with bilaminated and multilaminated membranes. These sacs had multiple 
      twists at different intervals along their length forming a chain of vesicles. The 
      development and maturation of the virus were observed in these vesicles. A number 
      of virus-containing vesicles were also present extracellularly in the debris of 
      lysed cells. In addition, virus was observed in layers of membranous cisternae 
      closely associated with vacuoles and plasma membrane. Some of the cisternae 
      opened to the extracellular space and appeared to allow the release of the virus. 
      Virus particles were also found in patches and in crystals within the cytoplasmic 
      matrix. Many lysed cells contained fibrils often associated with patches of 
      ferritin-tagged virus. This study presents morphological evidence for the release 
      of the virus in vesicles after cell lysis, via cisternae with openings to the 
      extracellular space, and in cytoplasmic blebs.
FAU - Yilma, T
AU  - Yilma T
FAU - Breese, S S
AU  - Breese SS
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cell Line
MH  - Cytoplasm/*microbiology
MH  - Enterovirus/*growth & development/ultrastructure
MH  - Inclusion Bodies, Viral/ultrastructure
MH  - Intracellular Membranes/microbiology/ultrastructure
MH  - Microscopy, Electron
MH  - Morphogenesis
MH  - Organoids/microbiology
EDAT- 1980/07/01 00:00
MHDA- 1980/07/01 00:01
CRDT- 1980/07/01 00:00
PHST- 1980/07/01 00:00 [pubmed]
PHST- 1980/07/01 00:01 [medline]
PHST- 1980/07/01 00:00 [entrez]
AID - 10.1099/0022-1317-49-1-225 [doi]
PST - ppublish
SO  - J Gen Virol. 1980 Jul;49(1):225-30. doi: 10.1099/0022-1317-49-1-225.