PMID- 6254008
OWN - NLM
STAT- MEDLINE
DCOM- 19810129
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 77
IP  - 7
DP  - 1980 Jul
TI  - Nucleotide sequence of the bacterial transposon Tn1681 encoding a heat-stable 
      (ST) toxin and its identification in enterotoxigenic Escherichia coli strains.
PG  - 4011-5
AB  - the Escherichia coli heat-stable toxin (ST I) is encoded within a transposon 
      (Tn1681) flanked by inverted repeats of insertion sequence 1 (IS1) [So, M., 
      Heffron, F. & McCarthy, B. J. (1979) Nature (London) 277, 453-456]. By subcloning 
      restriction fragments and by insertion mutagenesis, we located precisely the gene 
      for ST I within the transposon. We determined the complete nucleotide sequence of 
      the central portion of Tn1681 (i.e., that part flanked by IS1) and identified the 
      coding sequence of the toxin. From the nucleotide sequence, we deduced a probable 
      amino acid sequence for ST I. The NH2-terminal portion of the amino acid sequence 
      is extremely hydrophobic and bears a striking resemblance to the signal sequence 
      of the fd phage minor coat protein. By using a subcloned restriction fragment 
      containing the gene for ST I but no IS1 sequences, we determined (i) that the ST 
      toxin with activity assayable in suckling mice (ST I) is genetically distinct 
      from the St toxin assayable in ligated ileal loops (ST II) and (ii) that ST I can 
      be responsible for diarrheal disease in different animals.
FAU - So, M
AU  - So M
FAU - McCarthy, B J
AU  - McCarthy BJ
LA  - eng
SI  - GENBANK/J01831
PT  - Journal Article
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Bacterial Toxins)
RN  - 0 (DNA Transposable Elements)
RN  - 0 (DNA, Recombinant)
RN  - 0 (Enterotoxins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Amino Acid Sequence
MH  - Bacterial Toxins/*genetics
MH  - Base Sequence
MH  - Cloning, Molecular/methods
MH  - *DNA Transposable Elements
MH  - DNA, Recombinant
MH  - Enterotoxins/*genetics
MH  - Escherichia coli/*genetics
MH  - Hot Temperature
MH  - Nucleic Acid Conformation
MH  - RNA, Messenger/genetics
PMC - PMC349758
EDAT- 1980/07/01 00:00
MHDA- 1980/07/01 00:01
PMCR- 1981/01/01
CRDT- 1980/07/01 00:00
PHST- 1980/07/01 00:00 [pubmed]
PHST- 1980/07/01 00:01 [medline]
PHST- 1980/07/01 00:00 [entrez]
PHST- 1981/01/01 00:00 [pmc-release]
AID - 10.1073/pnas.77.7.4011 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1980 Jul;77(7):4011-5. doi: 10.1073/pnas.77.7.4011.