PMID- 6259003
OWN - NLM
STAT- MEDLINE
DCOM- 19810528
LR  - 20190813
IS  - 0303-7207 (Print)
IS  - 0303-7207 (Linking)
VI  - 21
IP  - 1
DP  - 1981 Jan
TI  - Guanine nucleotides modulate TRH-receptor binding in sheep anterior pituitary.
PG  - 85-91
AB  - Binding of thyrotropin-releasing hormone (TRH, thyroliberin) to receptors in 
      chilled membrane resuspensions from sheep anterior pituitary glands is reduced in 
      affinity almost 2-fold by micromolar concentrations of guanosine 5'-triphosphate 
      (GTP) and the hydrolysis-resistant analog guanyl-5'-yl imidodiphosphate (GppNHp) 
      added to tissue during a 10-min preincubation at 37 degree C. Guanosine 
      5'-diphosphate (GDP) produced a similar effect at 1 mM, while GMP, ATP, ADP and 
      AMP appeared relatively inactive. The number of TRH-binding sites was not 
      affected by the nucleotide treatments. These results are consistent with reports 
      of guanine nucleotide effects on other receptor types and with evidence 
      suggesting an adenylate cyclase mechanism for some of TRH's effects in the 
      anterior pituitary.
FAU - Taylor, R L
AU  - Taylor RL
FAU - Burt, D R
AU  - Burt DR
LA  - eng
GR  - MH 29671/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (Adenine Nucleotides)
RN  - 0 (Guanine Nucleotides)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Thyrotropin-Releasing Hormone)
RN  - 146-91-8 (Guanosine Diphosphate)
RN  - 34273-04-6 (Guanylyl Imidodiphosphate)
RN  - 5Y5F15120W (Thyrotropin-Releasing Hormone)
RN  - 85-32-5 (Guanosine Monophosphate)
RN  - 86-01-1 (Guanosine Triphosphate)
SB  - IM
MH  - Adenine Nucleotides/pharmacology
MH  - Animals
MH  - Guanine Nucleotides/*pharmacology
MH  - Guanosine Diphosphate/pharmacology
MH  - Guanosine Monophosphate/pharmacology
MH  - Guanosine Triphosphate/pharmacology
MH  - Guanylyl Imidodiphosphate/pharmacology
MH  - Pituitary Gland, Anterior/drug effects/*metabolism
MH  - Receptors, Cell Surface/drug effects/*metabolism
MH  - Receptors, Thyrotropin-Releasing Hormone
MH  - Sheep
MH  - Thyrotropin-Releasing Hormone/*metabolism
EDAT- 1981/01/01 00:00
MHDA- 1981/01/01 00:01
CRDT- 1981/01/01 00:00
PHST- 1981/01/01 00:00 [pubmed]
PHST- 1981/01/01 00:01 [medline]
PHST- 1981/01/01 00:00 [entrez]
AID - 10.1016/0303-7207(81)90033-2 [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 1981 Jan;21(1):85-91. doi: 10.1016/0303-7207(81)90033-2.