PMID- 6260345
OWN - NLM
STAT- MEDLINE
DCOM- 19810625
LR  - 20031114
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 41
IP  - 5
DP  - 1981 May
TI  - Changes in retrovirus expression in mouse mammary tumor cells in culture.
PG  - 1579-84
AB  - Mouse mammary tumor cells (Mm5Mt/c1) produced mouse mammary tumor virus (MMTV) 
      (type B retrovirus) at early passages in culture. They apparently produced 
      predominently type C retrovirus(es) at late passages in culture. The decline in 
      MMTV production during passage was not accompanied by an apparent decrease in the 
      synthesis or accumulation of intracellular MMTV-specific RNA. As judged by the 
      concentration of RNA (mol/titer) x half-time (sec) of hybridization of cellular 
      RNA with MMTV complementary DNA, the intracellular concentration MMTV-specific 
      RNA did not change significantly during passage in culture. Since late-passage 
      RNA, particularly polysomal poly(adenylic acid)-containing RNA, yielded lower 
      apparent maximum hybridization of MMTV complementary DNA as compared with 
      early-passage RNA, it was possible that the complexity or base sequence of 
      MMTV-specific RNA in late-passage cultures was not identical with that in 
      early-passage cultures. The data on thermal transitions of hybrids were in accord 
      with this possibility. In contrast, the steady-state level of type C 
      virus-specific RNA in late-passage cultures was orders of magnitude higher than 
      that in early-passage cultures. The production of type C retrovirus(es) at later 
      passages was apparently accompanied by increased transcription of type c 
      retrovirus DNA.
FAU - Arya, S K
AU  - Arya SK
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Extracellular Space/microbiology
MH  - Female
MH  - Gene Expression Regulation
MH  - *Genes, Viral
MH  - Mammary Neoplasms, Experimental/genetics/*microbiology
MH  - Mammary Tumor Virus, Mouse/*genetics
MH  - Mice
MH  - Polyribosomes/metabolism
MH  - RNA, Viral/metabolism
MH  - Retroviridae/*genetics
MH  - Transcription, Genetic
MH  - Virus Replication
EDAT- 1981/05/01 00:00
MHDA- 1981/05/01 00:01
CRDT- 1981/05/01 00:00
PHST- 1981/05/01 00:00 [pubmed]
PHST- 1981/05/01 00:01 [medline]
PHST- 1981/05/01 00:00 [entrez]
PST - ppublish
SO  - Cancer Res. 1981 May;41(5):1579-84.