PMID- 6269731
OWN - NLM
STAT- MEDLINE
DCOM- 19811222
LR  - 20071114
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 41
IP  - 10
DP  - 1981 Oct
TI  - Quantitation of viral antigens released into plasma and culture fluids by murine 
      mammary tumor cells.
PG  - 3885-90
AB  - Radioimmunoassay capable of measuring mouse mammary tumor virus (MMTV) 52,000 
      M.W. envelope glycoprotein (gp52) and 27,000 M.W. protein (p27) have been used to 
      quantitatively compare plasma concentrations of these viral antigens in mice 
      bearing spontaneous mammary tumors. Although gp52 was detected in the plasma of 
      all tumor-bearing mice tested, p27 was detected in only a portion of 
      tumor-bearing animals. In p27-positive animals, gp52 was detected in higher 
      concentrations than p27. These findings demonstrate that gp52 has preferential 
      utility as a plasma marker for the presence of mammary tumors in MMTV-infected 
      hybrid (BALB/c x DBA/8 F1), Paris RIII, and C3H/HeJ mice. In addition, cultures 
      of MMTV-producing cells [GR-MMTV and MMTV(C3H)Fel I] were used as models to study 
      the release of viral antigens in the absence of serum antibody or additional host 
      factors. Comparisons of extracellular soluble and particulate antigen 
      concentrations demonstrated that gp52 and p27 were present in substantially 
      higher concentrations as soluble than virion-associated antigens. The mean ratio 
      of non-virion-associated gp52 to virion-associated gp52 was 12.5:1 for GR-MMTV 
      cells and 37.3:1 for MMTV(C3H)Fel I cells. The marked stability of MMTV in 
      culture fluids suggested that virion breakdown was not responsible for the 
      accumulation of soluble viral antigens in culture. The information obtained 
      suggests that abundant virus-free antigens may be of greater use than 
      virion-associated antigens as a source of viral antigen to evaluate mammary tumor 
      status.
FAU - Ritzi, E M
AU  - Ritzi EM
FAU - Smith, B A
AU  - Smith BA
FAU - Stolfi, R L
AU  - Stolfi RL
FAU - Martin, D S
AU  - Martin DS
LA  - eng
GR  - 1 POL CA-25842/CA/NCI NIH HHS/United States
GR  - CA-28305-01/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 0 (Antigens, Viral)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Adenocarcinoma/*immunology
MH  - Animals
MH  - Antigens, Viral/*analysis
MH  - Cell Line
MH  - Female
MH  - Mammary Neoplasms, Experimental/*immunology
MH  - Mammary Tumor Virus, Mouse/*immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred Strains
MH  - Radioimmunoassay
MH  - Viral Proteins/analysis
EDAT- 1981/10/01 00:00
MHDA- 1981/10/01 00:01
CRDT- 1981/10/01 00:00
PHST- 1981/10/01 00:00 [pubmed]
PHST- 1981/10/01 00:01 [medline]
PHST- 1981/10/01 00:00 [entrez]
PST - ppublish
SO  - Cancer Res. 1981 Oct;41(10):3885-90.