PMID- 6273791 OWN - NLM STAT- MEDLINE DCOM- 19820212 LR - 20190501 IS - 0305-1048 (Print) IS - 1362-4962 (Electronic) IS - 0305-1048 (Linking) VI - 9 IP - 19 DP - 1981 Oct 10 TI - The endogenous proviral mouse mammary tumor virus genes of the GR mouse are not identical and only one corresponds to the exogenous virus. PG - 4981-95 AB - The endogenous proviral copies of mouse mammary tumor virus (MMTV) were selected from a gene library of GR mouse DNA. We obtained five different lambda. MMTV recombinant clones. Four of them correspond to the 3' Eco RI fragments of the endogenous proviruses an one comprises an intact MMTV provirus with 2 to 3 kb of flanking mouse genomic DNA. Heteroduplex formation followed by S1 digestion under stringent conditions shows that there is nucleotide sequence heterology among the cloned endogenous proviral copies. Only one endogenous proviral copy, associated with the mtv-2 locus, was found to be totally homologous to the exogenous proviral DNA. FAU - Herrlich, P AU - Herrlich P FAU - Hynes, N E AU - Hynes NE FAU - Ponta, H AU - Ponta H FAU - Rahmsdorf, U AU - Rahmsdorf U FAU - Kennedy, N AU - Kennedy N FAU - Groner, B AU - Groner B LA - eng PT - Journal Article PL - England TA - Nucleic Acids Res JT - Nucleic acids research JID - 0411011 RN - 0 (DNA, Viral) RN - EC 3.1.- (Endonucleases) RN - EC 3.1.21.- (DNA Restriction Enzymes) RN - EC 3.1.21.- (Deoxyribonuclease EcoRI) RN - EC 3.1.30.1 (Single-Strand Specific DNA and RNA Endonucleases) SB - IM MH - Animals MH - Cloning, Molecular MH - DNA Restriction Enzymes/metabolism MH - DNA, Viral/*analysis MH - Deoxyribonuclease EcoRI MH - Electrophoresis, Agar Gel MH - Endonucleases/metabolism MH - Mammary Tumor Virus, Mouse/*genetics MH - Mice MH - Single-Strand Specific DNA and RNA Endonucleases PMC - PMC327493 EDAT- 1981/10/10 00:00 MHDA- 1981/10/10 00:01 PMCR- 1981/10/10 CRDT- 1981/10/10 00:00 PHST- 1981/10/10 00:00 [pubmed] PHST- 1981/10/10 00:01 [medline] PHST- 1981/10/10 00:00 [entrez] PHST- 1981/10/10 00:00 [pmc-release] AID - 10.1093/nar/9.19.4981 [doi] PST - ppublish SO - Nucleic Acids Res. 1981 Oct 10;9(19):4981-95. doi: 10.1093/nar/9.19.4981.