PMID- 6274795
OWN - NLM
STAT- MEDLINE
DCOM- 19820326
LR  - 20181113
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 44
IP  - 4
DP  - 1981 Dec
TI  - Polymorphonuclear leucocyte membrane-stimulated oxidative metabolic 
      activity---the effect of divalent cations and cytochalasins.
PG  - 847-58
AB  - The effect of divalent cations and cytochalasins on the oxidative metabolic 
      response of polymorphonuclear leucocytes (PMNL) following membrane stimulation by 
      opsonized zymosan, phorbol myristate acetate (PMA), digitonin and 
      n-formyl-methionyl-leucyl-phenylalanine (FMLP) was investigated using several 
      techniques. For optimal ferricytochrome C reduction, oxygen consumption, luminol- 
      and lucigenin-dependent chemiluminescence by PMNL on exposure to opsonized 
      zymosan, extracellular magnesium was necessary. In contrast FMLP- and 
      digitonin-induced PMNL metabolic activities were less dependent on extracellular 
      magnesium than on calcium. Phorbol myristate acetate-induced PMNL oxidative 
      metabolic activity occurred independently of extracellular magnesium and calcium 
      concentration. Incubation of PMNL with cytochalasins B and E had little effect on 
      PMA-induced metabolic activity, enhanced FMLP-induced metabolic activity but 
      inhibited digitonin-induced metabolic activity. Investigation of zymosan-induced 
      PMNL metabolic activity after incubation with cytochalasins demonstrated a 
      dissociation between the different parameters of metabolic activity measured. The 
      results described in this study support the suggestion that PMNL oxidative 
      metabolism can be activated by at least two different mechanisms depending on the 
      stimulus used, and that these mechanisms can in part be differentiated by their 
      dependence on extracellular divalent cations.
FAU - Williams, A J
AU  - Williams AJ
FAU - Cole, P J
AU  - Cole PJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Cytochalasins)
RN  - 0 (Cytochrome c Group)
RN  - 0 (Oligopeptides)
RN  - 4289-98-9 (N-Formylmethionine)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 9010-72-4 (Zymosan)
RN  - I38ZP9992A (Magnesium)
RN  - KOO5CM684H (Digitonin)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adult
MH  - Calcium/*pharmacology
MH  - Cell Membrane/drug effects
MH  - Cells, Cultured
MH  - Cytochalasins/*pharmacology
MH  - Cytochrome c Group/metabolism
MH  - Digitonin/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Humans
MH  - Luminescent Measurements
MH  - Magnesium/*pharmacology
MH  - N-Formylmethionine/analogs & derivatives/pharmacology
MH  - N-Formylmethionine Leucyl-Phenylalanine
MH  - Neutrophils/*metabolism
MH  - Oligopeptides/pharmacology
MH  - Oxygen Consumption/*drug effects
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Zymosan/pharmacology
PMC - PMC1554984
EDAT- 1981/12/01 00:00
MHDA- 1981/12/01 00:01
PMCR- 1982/12/01
CRDT- 1981/12/01 00:00
PHST- 1981/12/01 00:00 [pubmed]
PHST- 1981/12/01 00:01 [medline]
PHST- 1981/12/01 00:00 [entrez]
PHST- 1982/12/01 00:00 [pmc-release]
PST - ppublish
SO  - Immunology. 1981 Dec;44(4):847-58.