PMID- 6277735 OWN - NLM STAT- MEDLINE DCOM- 19820521 LR - 20190707 IS - 0378-1119 (Print) IS - 0378-1119 (Linking) VI - 15 IP - 4 DP - 1981 Dec TI - Structure, stability, methylation, expression and glucocorticoid induction of endogenous and transfected proviral genes of mouse mammary tumor virus in mouse fibroblasts. PG - 307-17 AB - A sequence of mouse genomic DNA containing an endogenous mouse mammary tumor virus (MMTV) provirus, which was isolated by molecular cloning in lambda vector phage, has been reintroduced into cultured mouse L cells, using the thymidine-kinase cotransfection technique. Individual cell clones that acquired the transfected MMTV proviral DNA have been isolated. The transfected DNA remains stable in these cells and does not become methylated. It is transcribed into MMTV RNA, And the transcription is stimulated by glucocorticoid hormones. FAU - Hynes, N E AU - Hynes NE FAU - Rahmsdorf, U AU - Rahmsdorf U FAU - Kennedy, N AU - Kennedy N FAU - Fabiani, L AU - Fabiani L FAU - Michalides, R AU - Michalides R FAU - Nusse, R AU - Nusse R FAU - Groner, B AU - Groner B LA - eng PT - Journal Article PL - Netherlands TA - Gene JT - Gene JID - 7706761 RN - 0 (DNA, Viral) RN - 0 (Glucocorticoids) SB - IM MH - Animals MH - Cell Line MH - DNA, Viral/*genetics/metabolism MH - Glucocorticoids/*pharmacology MH - Mammary Tumor Virus, Mouse/*genetics MH - Methylation MH - Mice MH - Transcription, Genetic/*drug effects MH - Transfection EDAT- 1981/12/01 00:00 MHDA- 1981/12/01 00:01 CRDT- 1981/12/01 00:00 PHST- 1981/12/01 00:00 [pubmed] PHST- 1981/12/01 00:01 [medline] PHST- 1981/12/01 00:00 [entrez] AID - 0378-1119(81)90174-8 [pii] AID - 10.1016/0378-1119(81)90174-8 [doi] PST - ppublish SO - Gene. 1981 Dec;15(4):307-17. doi: 10.1016/0378-1119(81)90174-8.