PMID- 6281457
OWN - NLM
STAT- MEDLINE
DCOM- 19820722
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 41
IP  - 2
DP  - 1982 Feb
TI  - Processing and amino acid sequence analysis of the mouse mammary tumor virus env 
      gene product.
PG  - 414-22
AB  - The envelope proteins of mouse mammary tumor virus (MMTV) are synthesized from a 
      subgenomic 24S mRNA as a 75,000-dalton glycosylated precursor polyprotein which 
      is eventually processed to the mature glycoproteins gp52 and gp36. In vivo 
      synthesis of this env precursor in the presence of the core glycosylation 
      inhibitor tunicamycin yielded a precursor of approximately 61,000 daltons 
      (P61env). However, a 67,000-dalton protein (P67env) was obtained from cell-free 
      translation with the MMTV 24S mRNA as the template. To determine whether the 
      portion of the protein cleaved from P67env to give P61env was removed from the 
      NH2-terminal end of P67env and as such would represent a leader sequence, the 
      NH2-terminal amino acid sequence of the terminal peptide gp52 was determined. 
      Glutamic acid, and not methionine, was found to be the amino-terminal residue of 
      gp52, indicating that the cleaved portion was derived from the NH2-terminal end 
      of P67env. The NH2-terminal amino acid sequences of gp52's from endogenous and 
      exogenous C3H MMTVs were determined though 46 residues and found to be identical. 
      However, amino acid composition and type-specific gp52 radioimmunoassays from 
      MMTVs grown in heterologous cells indicated primary structure differences between 
      gp52's of the two viruses. The nucleic acid sequence of cloned MMTV DNA fragments 
      (J. Majors and H. E. Varmus, personal communication) in conjunction with the 
      NH2-terminal sequence of gp52 allowed localization of the env gene in the MMTV 
      genome. Nucleotides coding for the NH2 terminus of gp52 begin approximately 0.8 
      kilobase to the 3' side of the single EcoRI cleavage site. Localization of the 
      env gene at that point agrees with the proposed gene order -gag-pol-env- and also 
      allows sufficient coding potential for the glycoprotein precursor without 
      extending into the long terminal repeat.
FAU - Arthur, L O
AU  - Arthur LO
FAU - Copeland, T D
AU  - Copeland TD
FAU - Oroszlan, S
AU  - Oroszlan S
FAU - Schochetman, G
AU  - Schochetman G
LA  - eng
GR  - N01 CO-75380/CO/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Antigens, Viral)
RN  - 0 (Glycoproteins)
RN  - 0 (Protein Precursors)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, Viral/analysis
MH  - Female
MH  - *Genes, Viral
MH  - Glycoproteins/analysis
MH  - Mammary Tumor Virus, Mouse/*analysis
MH  - Mice
MH  - Molecular Weight
MH  - Protein Precursors/metabolism
MH  - Viral Envelope Proteins
MH  - Viral Proteins/*analysis/immunology
PMC - PMC256771
EDAT- 1982/02/01 00:00
MHDA- 1982/02/01 00:01
PMCR- 1982/02/01
CRDT- 1982/02/01 00:00
PHST- 1982/02/01 00:00 [pubmed]
PHST- 1982/02/01 00:01 [medline]
PHST- 1982/02/01 00:00 [entrez]
PHST- 1982/02/01 00:00 [pmc-release]
AID - 10.1128/JVI.41.2.414-422.1982 [doi]
PST - ppublish
SO  - J Virol. 1982 Feb;41(2):414-22. doi: 10.1128/JVI.41.2.414-422.1982.