PMID- 6282137
OWN - NLM
STAT- MEDLINE
DCOM- 19820708
LR  - 20171213
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 242
IP  - 5
DP  - 1982 May
TI  - Glucose-induced proton uptake in secretory granules of beta-cells in monolayer 
      culture.
PG  - C382-7
AB  - The weak base acridine orange (AO) has been shown to be accumulated by the 
      insulin-containing secretory granules of cultured beta-cells in response to high 
      glucose. Various lines of evidence indicate that this accumulation is due to a pH 
      gradient. Thus ionophores such as monensin and nigericin abolish the 
      glucose-induced accumulation, and a high concentration of the weak base, 
      benzylamine, results in swelling of the granules. In the absence of glucose, ATP 
      addition to digitonin-permeabilized cells also results in dye uptake. These data 
      also suggest that a primary or secondary active accumulation mechanism for 
      hydrogen ions exists across the granule membrane.
FAU - Pace, C S
AU  - Pace CS
FAU - Sachs, G
AU  - Sachs G
LA  - eng
GR  - AM-00499/AM/NIADDK NIH HHS/United States
GR  - AM-21973/AM/NIADDK NIH HHS/United States
GR  - AM-28459/AM/NIADDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Protons)
RN  - F30N4O6XVV (Acridine Orange)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - Cells, Cultured
MH  - Cytoplasmic Granules/*metabolism
MH  - Glucose/*pharmacology
MH  - Hydrogen-Ion Concentration
MH  - Islets of Langerhans/*metabolism
MH  - Male
MH  - *Protons
MH  - Rats
MH  - Rats, Inbred Strains
EDAT- 1982/05/01 00:00
MHDA- 1982/05/01 00:01
CRDT- 1982/05/01 00:00
PHST- 1982/05/01 00:00 [pubmed]
PHST- 1982/05/01 00:01 [medline]
PHST- 1982/05/01 00:00 [entrez]
AID - 10.1152/ajpcell.1982.242.5.C382 [doi]
PST - ppublish
SO  - Am J Physiol. 1982 May;242(5):C382-7. doi: 10.1152/ajpcell.1982.242.5.C382.