PMID- 6287686 OWN - NLM STAT- MEDLINE DCOM- 19821029 LR - 20190511 IS - 0035-9203 (Print) IS - 0035-9203 (Linking) VI - 76 IP - 3 DP - 1982 TI - A zymodeme study of Entamoeba histolytica in a group of South African schoolchildren. PG - 401-2 AB - Using a biphasic culture medium, stocks of intestinal amoebae were isolated from a group of children attending school in Durban, South Africa. These were compared with stocks collected in other areas of the world already characterized using the electrophoretic patterns of four enzymes: glucose phosphate isomerase (GPI), phosphoglucomutase (PGM) L-malate: NADP+ oxido-reductase (oxalacetate-decarboxylating) (ME) and hexokinase (HK). 33% of 94 samples grew Entamoeba histolytica, only one of which gave a pattern indicative of a pathogenic stock. Entamoeba hartmanni, Dientamoeba fragilis and Entamoeba coli were also grown from some samples, increasing the total positive samples for all species isolated to 40%. FAU - Sargeaunt, P G AU - Sargeaunt PG FAU - Williams, J E AU - Williams JE FAU - Jackson, T F AU - Jackson TF FAU - Simjee, A E AU - Simjee AE LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - England TA - Trans R Soc Trop Med Hyg JT - Transactions of the Royal Society of Tropical Medicine and Hygiene JID - 7506129 RN - 0 (Isoenzymes) SB - IM MH - Adolescent MH - Amebiasis/*parasitology MH - Child MH - Dientamoeba/enzymology MH - Entamoeba/enzymology MH - Entamoeba histolytica/*enzymology MH - Entamoebiasis/*parasitology MH - Humans MH - Isoenzymes/*analysis EDAT- 1982/01/01 00:00 MHDA- 1982/01/01 00:01 CRDT- 1982/01/01 00:00 PHST- 1982/01/01 00:00 [pubmed] PHST- 1982/01/01 00:01 [medline] PHST- 1982/01/01 00:00 [entrez] AID - 10.1016/0035-9203(82)90200-0 [doi] PST - ppublish SO - Trans R Soc Trop Med Hyg. 1982;76(3):401-2. doi: 10.1016/0035-9203(82)90200-0.