PMID- 6289701
OWN - NLM
STAT- MEDLINE
DCOM- 19821202
LR  - 20190628
IS  - 0003-3022 (Print)
IS  - 0003-3022 (Linking)
VI  - 57
IP  - 4
DP  - 1982 Oct
TI  - Nitroprusside increases cyclic guanylate monophosphate concentrations during 
      relaxation of rabbit aortic strips and both effects are antagonized by cyanide.
PG  - 303-8
AB  - The authors have confirmed previous observations that sodium cyanide (CN-) 
      partially reverses the vasodilator effects of sodium nitroprusside (SNP) on 
      vascular smooth muscle. As tested on rabbit aortic strips contracted by 
      norepinephrine (NE), the final tension is independent of the order of addition of 
      reagents. In the same concentration, CN- alone had no effect on tension also as 
      reported by others. The ED50 values for relaxation of aortic strips for a series 
      of directly acting agonists ("nitric oxide vasodilators") were: sodium azide 
      (N-3) 2.1 X 10(-7) M; SNP 2.7 X 10(-7) M; hydroxylamine (H2NOH) hydrochloride 2.5 
      X 10(-6) M; human nitric oxide hemoglobin (HbNO) 3.5 X 10(-6) M; and sodium 
      nitrite (NO-2) 1.2 X 10(-4) M. In addition to SNP, CN- antagonized the 
      vasodilator effects of N-3 and H2NOH, but it failed to reverse relaxation by 
      HbNO, NO gas, NO-2 (as observed by us), glyceryl trinitrate, adenosine, or 
      papaverine (as observed by others). The only change noted in cyclic-adenosine 
      monophosphate (c-AMP) concentrations in aortic strips exposed to 1) NE, 2) NE + 
      NO-2 or SNP, or 3) NE + NO-2 or SNP + CN- was an increase due to NE. The only 
      statistically significant change noted in cyclic-guanosine monophosphate (c-GMP) 
      concentrations exposed to 1) NE, 2) NE + NO-2 or 3) NE + NO-2 + CN- was also an 
      increase due to NE. In contrast, SNP resulted in further increases in c-GMP after 
      NE, and when cyanide was added, a significant decrease in c-GMP followed. These 
      results are only partially consistent with a role for c-GMP in relaxation of 
      vascular smooth muscle, but cyanide may become a useful tool for the study of 
      mechanisms of action of the nitric oxide vasodilators.
FAU - Kruszyna, H
AU  - Kruszyna H
FAU - Kruszyna, R
AU  - Kruszyna R
FAU - Smith, R P
AU  - Smith RP
LA  - eng
GR  - HL 14127/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Anesthesiology
JT  - Anesthesiology
JID - 1300217
RN  - 0 (Cyanides)
RN  - 0 (Ferricyanides)
RN  - 0 (Vasodilator Agents)
RN  - 169D1260KM (Nitroprusside)
RN  - H2D2X058MU (Cyclic GMP)
SB  - IM
MH  - Animals
MH  - Aorta, Thoracic/drug effects
MH  - Cyanides/*pharmacology
MH  - Cyclic GMP/*metabolism
MH  - Ferricyanides/*pharmacology
MH  - In Vitro Techniques
MH  - Muscle Contraction/*drug effects
MH  - Muscle Relaxation/*drug effects
MH  - Muscle, Smooth, Vascular/*drug effects/metabolism
MH  - Nitroprusside/antagonists & inhibitors/*pharmacology
MH  - Rabbits
MH  - Vasodilator Agents/antagonists & inhibitors/pharmacology
EDAT- 1982/10/01 00:00
MHDA- 1982/10/01 00:01
CRDT- 1982/10/01 00:00
PHST- 1982/10/01 00:00 [pubmed]
PHST- 1982/10/01 00:01 [medline]
PHST- 1982/10/01 00:00 [entrez]
AID - 10.1097/00000542-198210000-00010 [doi]
PST - ppublish
SO  - Anesthesiology. 1982 Oct;57(4):303-8. doi: 10.1097/00000542-198210000-00010.