PMID- 6296429
OWN - NLM
STAT- MEDLINE
DCOM- 19830311
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 45
IP  - 1
DP  - 1983 Jan
TI  - Cloned mouse mammary tumor virus DNA exhibits glucocorticoid-dependent expression 
      in simian virus 40-transformed mink cells.
PG  - 148-54
AB  - Mink lung epithelial cells were transfected with two cloned mouse mammary tumor 
      virus (MMTV) DNAs, a 9-kilobase clone derived from an unintegrated exogenous 
      viral genome and a 14-kilobase clone containing an integrated endogenous provirus 
      along with cellular flanking sequences. Mink lung cells were chosen because they 
      do not contain endogenous MMTV sequences. On the basis of our observation that 
      simian virus 40 DNA efficiently transforms these cells, we isolated cell clones 
      containing MMTV DNA by using transformation with simian virus 40 DNA as a 
      selective marker in cotransfection experiments. Levels of the 9-kilobase MMTV 
      mRNA representing the entire viral genome and of the spliced 4.4-kilobase mRNA 
      which codes for the viral envelope proteins were glucocorticoid dependent in 
      transformed cells. Expression of low levels of Pr77gag, the precursor of the 
      group-specific viral core proteins, and of gPr73env, the precursor of the viral 
      envelope proteins, was also hormone dependent. We conclude that these cloned MMTV 
      DNAs contain all the information necessary for the synthesis of normal viral RNAs 
      and proteins. These findings also provide further evidence that the DNA sequences 
      involved in the hormone responsiveness of MMTV expression are contained within 
      the viral genome.
FAU - Owen, D
AU  - Owen D
FAU - Diggelmann, H
AU  - Diggelmann H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (DNA, Viral)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Core Proteins)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Proteins)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cell Transformation, Viral
MH  - Cloning, Molecular
MH  - DNA, Viral/genetics
MH  - Dexamethasone/*pharmacology
MH  - Gene Expression Regulation/*drug effects
MH  - Mammary Tumor Virus, Mouse/*genetics
MH  - Mink
MH  - RNA, Messenger/biosynthesis
MH  - RNA, Viral/biosynthesis
MH  - Simian virus 40/physiology
MH  - *Transfection
MH  - Viral Core Proteins
MH  - Viral Envelope Proteins
MH  - Viral Proteins/biosynthesis
PMC - PMC256396
EDAT- 1983/01/01 00:00
MHDA- 1983/01/01 00:01
PMCR- 1983/01/01
CRDT- 1983/01/01 00:00
PHST- 1983/01/01 00:00 [pubmed]
PHST- 1983/01/01 00:01 [medline]
PHST- 1983/01/01 00:00 [entrez]
PHST- 1983/01/01 00:00 [pmc-release]
AID - 10.1128/JVI.45.1.148-154.1983 [doi]
PST - ppublish
SO  - J Virol. 1983 Jan;45(1):148-54. doi: 10.1128/JVI.45.1.148-154.1983.