PMID- 6298716
OWN - NLM
STAT- MEDLINE
DCOM- 19830407
LR  - 20190501
IS  - 0305-1048 (Print)
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Linking)
VI  - 10
IP  - 24
DP  - 1982 Dec 20
TI  - A comparison of nucleoside (beta-S)triphosphates and nucleoside 
      (gamma-S)triphosphates as suitable substrates for measuring transcription 
      initiation in preparations of cell nuclei.
PG  - 8311-22
AB  - RNA chains initiated with nucleoside (beta-S)triphosphates and 
      (gamma-S)triphosphates retain the thiol groups and can be separated from 
      thiol-free RNA by chromatography on mercury-Sepharose. Thiol-containing mouse 
      mammary tumor virus (MMTV) RNA synthesized by preparations of nuclei from 
      virus-infected cells was quantitated by nucleic acid filter hybridization. With 
      ATP beta S and GTP beta S, region-specific initiation of MMTV RNA chains was 
      detected in the cell free system. However, with ATP gamma S and GTP gamma S, 
      region-specific initiation was not clearly demonstrable. The nuclear preparations 
      can also transfer thiol groups, presumably in the form of thiophosphate, from ATP 
      gamma S or GTP gamma S onto preexisting RNA molecules; little or no 
      thiol-transfer occurs with the two (beta-S)-analogues. The thiophosphate transfer 
      activity apparently interferes with the measurement of RNA chain initiation with 
      ATP gamma S and GTP gamma S.
FAU - Washington, L D
AU  - Washington LD
FAU - Stallcup, M R
AU  - Stallcup MR
LA  - eng
GR  - GM28298/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (RNA, Viral)
RN  - 0 (Thionucleosides)
RN  - 0 (Thionucleotides)
RN  - EC 2.7.7.6 (DNA-Directed RNA Polymerases)
SB  - IM
MH  - Animals
MH  - Cell Nucleus/drug effects/*metabolism
MH  - DNA-Directed RNA Polymerases/*metabolism
MH  - Escherichia coli/enzymology/genetics
MH  - Kinetics
MH  - Mammary Tumor Virus, Mouse/genetics
MH  - Mice
MH  - Nucleic Acid Hybridization
MH  - RNA, Viral/genetics
MH  - Structure-Activity Relationship
MH  - Thionucleosides/*pharmacology
MH  - Thionucleotides/*pharmacology
MH  - Transcription, Genetic/*drug effects
PMC - PMC327088
EDAT- 1982/12/20 00:00
MHDA- 1982/12/20 00:01
CRDT- 1982/12/20 00:00
PHST- 1982/12/20 00:00 [pubmed]
PHST- 1982/12/20 00:01 [medline]
PHST- 1982/12/20 00:00 [entrez]
AID - 10.1093/nar/10.24.8311 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 1982 Dec 20;10(24):8311-22. doi: 10.1093/nar/10.24.8311.