PMID- 6298918
OWN - NLM
STAT- MEDLINE
DCOM- 19830407
LR  - 20071115
IS  - 0034-5164 (Print)
IS  - 0034-5164 (Linking)
VI  - 38
IP  - 3
DP  - 1982 Dec
TI  - Taste bud adenosine -3'5'-monophosphate phosphodiesterase: activity, subcellular 
      distribution and kinetic parameters.
PG  - 439-52
AB  - Higher activity of cyclic adenosine 3',5'-monophosphate (cAMP) phosphodiesterase 
      (PDE) was found in homogenates from bovine circumvellate papillae bearing taste 
      buds compared to activity in homogenates from areas surrounding these papillae in 
      which no taste buds were present. With progressive purification of these 
      homogenates cAMP PDE activity increased in the taste bud enriched fractions 
      relative to that measured in the non-taste bud bearing epithelial tissue. The 
      highest levels of cAMP PDE activity were measured in those taste bud fractions in 
      which purification was greatest. Kinetic studies in both taste bud derived and 
      control tissues showed two apparent Km values, one relatively high, the other, 
      lower. cAMP PDE activity of taste bud membranes was enhanced by Mg++, Mn++ and 
      imidazole and inhibited by ethylene-bis (beta-aminoethylether) N,N-tetra-acetic 
      acid (EGTA), isobutyl methyl xanthine (IBMX), theophylline, and cyclic guanosine 
      3',5'-monophosphate (cGMP). The possible role for cAMP PDE in taste function is 
      discussed.
FAU - Law, J S
AU  - Law JS
FAU - Henkin, R I
AU  - Henkin RI
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Res Commun Chem Pathol Pharmacol
JT  - Research communications in chemical pathology and pharmacology
JID - 0244734
RN  - EC 3.1.4.17 (3',5'-Cyclic-AMP Phosphodiesterases)
SB  - IM
MH  - 3',5'-Cyclic-AMP Phosphodiesterases/*analysis/antagonists & inhibitors
MH  - Animals
MH  - Cattle
MH  - Kinetics
MH  - Taste Buds/*enzymology/ultrastructure
EDAT- 1982/12/01 00:00
MHDA- 1982/12/01 00:01
CRDT- 1982/12/01 00:00
PHST- 1982/12/01 00:00 [pubmed]
PHST- 1982/12/01 00:01 [medline]
PHST- 1982/12/01 00:00 [entrez]
PST - ppublish
SO  - Res Commun Chem Pathol Pharmacol. 1982 Dec;38(3):439-52.