PMID- 6301465
OWN - NLM
STAT- MEDLINE
DCOM- 19830527
LR  - 20190612
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 110
IP  - 3
DP  - 1983 Feb 10
TI  - A reevaluation of the chemotactic potency of leukotriene B4 (LTB4).
PG  - 842-50
AB  - The chemotactic potency of leukotriene B4 (LTB4) was reevaluated based on an 
      improved purification procedure which combines reversed phase and straight phase 
      high pressure liquid chromatography (RP-HPLC and SP-HPLC). Socalled LTB4 isomer 
      III prepared by RP-HPLC contains two double oxygenated 5,12-dihydroxy acids in 
      addition to LTB4. On a molar basis, the chemotactic activity of LTB4 repurified 
      by SP-HPLC was far greater than that of the other two 5,12-dihydroxy acids and 
      comparable to that of formyl-methionyl-leucyl-phenylalanine (fMLP). The 
      chemotactic activity of LTB4 isomer III is dependent upon the relative 
      concentrations of the double oxygenated 5,12-dihydroxy acids and LTB4. Further 
      purification of peak III by SP-HPLC is required before assessing the biologic 
      activity of LTB4.
FAU - Jubiz, W
AU  - Jubiz W
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Oligopeptides)
RN  - 1HGW4DR56D (Leukotriene B4)
RN  - 4289-98-9 (N-Formylmethionine)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
SB  - IM
MH  - Chemotaxis, Leukocyte/*drug effects
MH  - Chromatography, High Pressure Liquid
MH  - Gas Chromatography-Mass Spectrometry
MH  - Humans
MH  - Isomerism
MH  - Leukotriene B4/isolation & purification/*pharmacology
MH  - N-Formylmethionine/analogs & derivatives/pharmacology
MH  - N-Formylmethionine Leucyl-Phenylalanine
MH  - Neutrophils/drug effects/*physiology
MH  - Oligopeptides/pharmacology
MH  - Spectrophotometry, Ultraviolet
MH  - Structure-Activity Relationship
EDAT- 1983/02/10 00:00
MHDA- 1983/02/10 00:01
CRDT- 1983/02/10 00:00
PHST- 1983/02/10 00:00 [pubmed]
PHST- 1983/02/10 00:01 [medline]
PHST- 1983/02/10 00:00 [entrez]
AID - 0006-291X(83)91038-0 [pii]
AID - 10.1016/0006-291x(83)91038-0 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1983 Feb 10;110(3):842-50. doi: 
      10.1016/0006-291x(83)91038-0.