PMID- 6304568
OWN - NLM
STAT- MEDLINE
DCOM- 19830708
LR  - 20190712
IS  - 0306-4522 (Print)
IS  - 0306-4522 (Linking)
VI  - 8
IP  - 3
DP  - 1983 Mar
TI  - The corticopontine projection: axotomy-induced loss of high affinity L-glutamate 
      and D-aspartate uptake, but not of gamma-aminobutyrate uptake, glutamate 
      decarboxylase or choline acetyltransferase, in the pontine nuclei.
PG  - 449-57
AB  - The corticopontine fibres were severed in the crus cerebri in rats and mice by a 
      stereotaxically operated retractable wire-knife. The pontine nuclei were 
      microscopically dissected from fresh slices of rats and synaptosome-containing 
      homogenates were prepared. The high affinity uptake of radiolabelled L-glutamate 
      (L-Glu) and D-aspartate (D-Asp) was heavily reduced five days after the lesions. 
      The uptake was further reduced after bilateral (-75% for D-Asp and -65% for 
      L-Glu) than after unilateral lesions (-55% for D-Asp and -45 to 50% for L-Glu on 
      the lesioned side.) The molar ratio of the uptakes of D-Asp and L-Glu was 
      consistently lower in pons after transection of the cortical afferents than 
      normally (-28% after bilateral lesions). gamma-Aminobutyrate uptake and glutamic 
      acid decarboxylase were not changed. Choline acetyltransferase was increased 
      (+53%) after unilateral lesions, but not altered after bilateral lesions. 
      Autoradiograms of slices from mice, incubated with tritium-labelled amino acids 
      and fixed in glutaraldehyde, showed high affinity uptake sites for D-Asp to be 
      enriched in the pontine nuclei, compared to neighbouring structures. After 
      partial lesion of the crus cerebri the uptake was reduced in the area with 
      degenerated corticopontine afferents. gamma-Aminobutyrate uptake sites were 
      relatively less concentrated in the pontine nuclei than D-Asp uptake sites. The 
      results indicate, along with the previous demonstration of Ca-dependent K-induced 
      release of D-[3H]aspartate from the corticopontine terminals, that glutamate 
      and/or aspartate may be transmitters in this pathway. The results also suggest 
      that acidic amino acid uptake sites may differ in their relative transport rates 
      for aspartate and glutamate.
FAU - Thangnipon, W
AU  - Thangnipon W
FAU - Taxt, T
AU  - Taxt T
FAU - Brodal, P
AU  - Brodal P
FAU - Storm-Mathisen, J
AU  - Storm-Mathisen J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Glutamates)
RN  - 30KYC7MIAI (Aspartic Acid)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
RN  - EC 2.3.1.6 (Choline O-Acetyltransferase)
SB  - IM
MH  - Animals
MH  - Aspartic Acid/*metabolism
MH  - Autoradiography
MH  - Axons/*physiology
MH  - Cerebral Cortex/*physiology
MH  - Choline O-Acetyltransferase/*metabolism
MH  - Glutamates/*metabolism
MH  - Male
MH  - Pons/metabolism/*physiology
MH  - Rats
MH  - Rats, Inbred Strains
MH  - *Synaptic Transmission
MH  - gamma-Aminobutyric Acid/*metabolism
EDAT- 1983/03/01 00:00
MHDA- 1983/03/01 00:01
CRDT- 1983/03/01 00:00
PHST- 1983/03/01 00:00 [pubmed]
PHST- 1983/03/01 00:01 [medline]
PHST- 1983/03/01 00:00 [entrez]
AID - 0306-4522(83)90191-4 [pii]
AID - 10.1016/0306-4522(83)90191-4 [doi]
PST - ppublish
SO  - Neuroscience. 1983 Mar;8(3):449-57. doi: 10.1016/0306-4522(83)90191-4.