PMID- 6309675
OWN - NLM
STAT- MEDLINE
DCOM- 19831021
LR  - 20210526
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 41
IP  - 3
DP  - 1983 Sep
TI  - Stimulation of calcium uptake and cyclic GMP synthesis in rat basophilic leukemia 
      cells by Escherichia coli heat-stable enterotoxin.
PG  - 971-7
AB  - The effect of Escherichia coli heat-stable (ST) enterotoxin on calcium and cyclic 
      nucleotide metabolism in rat basophilic leukemia cell cultures was investigated. 
      Addition of ST enterotoxin to rat basophilic leukemia cell cultures resulted in 
      dose- and time-dependent stimulation of calcium uptake and elevation of the 
      intracellular cyclic GMP (cGMP) concentration. The effect of ST enterotoxin on 
      calcium uptake (P less than 0.02) and cGMP synthesis (P less than 0.02) was 
      demonstrated after 5 and 30 min of incubation at 37 degrees C, respectively. In 
      further studies ST enterotoxin did not enhance calcium release or the 
      intracellular concentration of cyclic AMP. The stimulation of calcium uptake and 
      cGMP synthesis by ST enterotoxin was inhibited by pharmacological and chemical 
      agents which block cellular calcium entry and prostaglandin synthesis. These 
      results demonstrate that ST enterotoxin induces calcium uptake and cGMP synthesis 
      in rat basophilic leukemia cell cultures.
FAU - Knoop, F C
AU  - Knoop FC
FAU - Thomas, D D
AU  - Thomas DD
LA  - eng
GR  - RR05390/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Enterotoxins)
RN  - H2D2X058MU (Cyclic GMP)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/*metabolism
MH  - Cells, Cultured
MH  - Cyclic GMP/*biosynthesis
MH  - Dose-Response Relationship, Drug
MH  - Drug Stability
MH  - Enterotoxins/*pharmacology
MH  - *Escherichia coli
MH  - Hot Temperature
MH  - Leukemia, Experimental/*metabolism
MH  - Rats
MH  - Stimulation, Chemical
MH  - Time Factors
PMC - PMC264596
EDAT- 1983/09/01 00:00
MHDA- 1983/09/01 00:01
PMCR- 1983/09/01
CRDT- 1983/09/01 00:00
PHST- 1983/09/01 00:00 [pubmed]
PHST- 1983/09/01 00:01 [medline]
PHST- 1983/09/01 00:00 [entrez]
PHST- 1983/09/01 00:00 [pmc-release]
AID - 10.1128/iai.41.3.971-977.1983 [doi]
PST - ppublish
SO  - Infect Immun. 1983 Sep;41(3):971-7. doi: 10.1128/iai.41.3.971-977.1983.