PMID- 6311347
OWN - NLM
STAT- MEDLINE
DCOM- 19831123
LR  - 20190614
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 273
IP  - 1
DP  - 1983 Aug 22
TI  - Tryptophan hydroxylase and 5-HTP-decarboxylase activity in cockroach brain and 
      the effects of p-chlorophenylalanine and 3-hydroxybenzylhydrazine (NSD-1015).
PG  - 67-70
AB  - The activities of two enzymes of the serotonin biosynthetic pathway, namely 
      tryptophan hydroxylase (TPH) and 5-hydroxytryptophan-decarboxylase (5-HTP-D), 
      were determined in the cockroach, Periplaneta americana, brain using radiometric 
      techniques, significant levels of both enzymes were found. p-Chlorophenylalanine 
      (PCPA) inhibited brain TPH activity strongly both in vitro and in vivo. In vitro, 
      10 mM PCPA reduced the TPH activity by 76% and in vivo either 15 micrograms/g or 
      30 micrograms/g of PCPA inhibited brain TPH activity by 80-85%. The activity of 
      5-HTP-D enzyme was inhibited significantly in vivo by 3-hydroxybenzylhydrazine, 
      commonly known as NSD-1015. A recovery in the activity of 5-HTP-D enzyme was 
      observed 12 h after 50 micrograms/g of NSD-1015 but 100 micrograms/g caused an 
      inhibition lasting for the 24 h period studied.
FAU - Pandey, A
AU  - Pandey A
FAU - Habibulla, M
AU  - Habibulla M
FAU - Singh, R
AU  - Singh R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Hydrazines)
RN  - 333DO1RDJY (Serotonin)
RN  - A27K5Q85R2 (3-hydroxybenzylhydrazine)
RN  - EC 1.14.16.4 (Tryptophan Hydroxylase)
RN  - EC 4.1.1.28 (Aromatic-L-Amino-Acid Decarboxylases)
RN  - R5J7E3L9SP (Fenclonine)
SB  - IM
MH  - Animals
MH  - Aromatic-L-Amino-Acid Decarboxylases/*metabolism
MH  - Cockroaches/*metabolism
MH  - Fenclonine/*pharmacology
MH  - Ganglia/*enzymology/metabolism
MH  - Hydrazines/*pharmacology
MH  - Periplaneta/*metabolism
MH  - Serotonin/biosynthesis
MH  - Synaptic Transmission
MH  - Tryptophan Hydroxylase/*metabolism
EDAT- 1983/08/22 00:00
MHDA- 1983/08/22 00:01
CRDT- 1983/08/22 00:00
PHST- 1983/08/22 00:00 [pubmed]
PHST- 1983/08/22 00:01 [medline]
PHST- 1983/08/22 00:00 [entrez]
AID - 0006-8993(83)91095-8 [pii]
AID - 10.1016/0006-8993(83)91095-8 [doi]
PST - ppublish
SO  - Brain Res. 1983 Aug 22;273(1):67-70. doi: 10.1016/0006-8993(83)91095-8.