PMID- 6312292
OWN - NLM
STAT- MEDLINE
DCOM- 19831123
LR  - 20210526
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 3
IP  - 8
DP  - 1983 Aug
TI  - Amplification and hormone-regulated expression of a mouse mammary tumor virus-Eco 
      gpt fusion plasmid in mouse 3T6 cells.
PG  - 1421-9
AB  - Mouse 3T6 cells were transformed with a chimeric DNA plasmid, pSVMgpt, in which 
      the mouse mammary tumor virus (MMTV) promoter was fused to the Escherichia coli 
      gene encoding xanthine-guanine phosphoribosyl transferase (Eco gpt). The 
      transformants exhibited glucocorticoid-inducible expression of Eco gpt. With 
      limiting xanthine concentrations, conditions were established in which cell 
      growth became hormone dependent. Cells selected for their ability to grow in 
      limiting concentrations of both xanthine and glucocorticoids contained amplified 
      levels of Eco gpt DNA, and expression of Eco gpt remained glucocorticoid 
      inducible in these amplified cells. Thus, amplification of the MMTV promoter 
      region in itself did not abolish hormonal responsiveness of a gene. In addition 
      to increased levels of Eco gpt DNA, some of the selected cells also exhibited 
      increased levels (two- to threefold) of glucocorticoid receptors. Lastly, we 
      found that excessive expression of Eco gpt is toxic to 3T6 cells; by maintaining 
      low hormone levels and, therefore, low levels of expression, we were able to 
      select cells with amplified Eco gpt. Thus, the MMTV promoter may be of general 
      utility in expressing genes whose products may be lethal if they are produced in 
      excessive quantities.
FAU - Chapman, A B
AU  - Chapman AB
FAU - Costello, M A
AU  - Costello MA
FAU - Lee, F
AU  - Lee F
FAU - Ringold, G M
AU  - Ringold GM
LA  - eng
GR  - GM07365/GM/NIGMS NIH HHS/United States
GR  - GM25821/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Glucocorticoids)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Glucocorticoid)
RN  - EC 2.4.2.- (Pentosyltransferases)
RN  - EC 2.4.2.22 (xanthine phosphoribosyltransferase)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - *Gene Amplification
MH  - Gene Expression Regulation/*drug effects
MH  - Glucocorticoids/*pharmacology
MH  - Mammary Tumor Virus, Mouse/*genetics
MH  - Mice
MH  - Pentosyltransferases/genetics
MH  - Plasmids
MH  - RNA, Messenger/biosynthesis
MH  - Receptors, Glucocorticoid/genetics
MH  - Repetitive Sequences, Nucleic Acid
PMC - PMC369988
EDAT- 1983/08/01 00:00
MHDA- 1983/08/01 00:01
PMCR- 1983/08/01
CRDT- 1983/08/01 00:00
PHST- 1983/08/01 00:00 [pubmed]
PHST- 1983/08/01 00:01 [medline]
PHST- 1983/08/01 00:00 [entrez]
PHST- 1983/08/01 00:00 [pmc-release]
AID - 10.1128/mcb.3.8.1421-1429.1983 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1983 Aug;3(8):1421-9. doi: 10.1128/mcb.3.8.1421-1429.1983.