PMID- 6315837
OWN - NLM
STAT- MEDLINE
DCOM- 19840107
LR  - 20141120
IS  - 0022-2143 (Print)
IS  - 0022-2143 (Linking)
VI  - 102
IP  - 6
DP  - 1983 Dec
TI  - Regulation of monocyte oxidative metabolism: chemotactic factor enhancement of 
      superoxide release, hydroxyl radical generation, and chemiluminescence.
PG  - 890-8
AB  - Human peripheral blood mononuclear cells exposed to the synthetic chemotactic 
      factor n-formyl-methionyl-leucyl-phenylalanine (FMLP) were enhanced in their 
      ability to generate superoxide anion (O-2), hydroxyl radical (OH.), and 
      chemiluminescence when later exposed to phorbol myristate acetate (PMA). When 
      compared to oxidative responses of cells treated with PMA alone, the degree of 
      enhancement by pretreatment with FMLP was 1.85-fold for O-2 generation, 1.73-fold 
      for OH. production, and 1.34-fold for chemiluminescence. Similarly, pretreatment 
      of mononuclear leukocytes with 5% zymosan-activated serum also enhanced 
      subsequent oxidative responses of cells exposed to PMA. FMLP did not enhance 
      subsequent O-2 release or chemiluminescence by mononuclear leukocytes stimulated 
      by opsonized zymosan or 20 mM sodium fluoride (F-), demonstrating that the O-2 
      generating system of monocytes stimulated by phagocytosis or F- is not 
      susceptible to chemotactic factor regulation in a manner similar to the system 
      stimulated by PMA. The latter system, like that of neutrophils, is susceptible to 
      regulation by cellular processes activated during an initial encounter with 
      chemoattractants. These processes may provide a mechanism to amplify oxidative 
      responses at sites of infection or inflammation, leading to enhanced efficiency 
      of microbicidal activity or increased tissue damage in vivo.
FAU - Janco, R L
AU  - Janco RL
FAU - English, D
AU  - English D
LA  - eng
GR  - RR 17950/RR/NCRR NIH HHS/United States
GR  - RR-05424-20/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Lab Clin Med
JT  - The Journal of laboratory and clinical medicine
JID - 0375375
RN  - 0 (Chemotactic Factors)
RN  - 0 (Free Radicals)
RN  - 0 (Hydroxides)
RN  - 11062-77-4 (Superoxides)
RN  - 3352-57-6 (Hydroxyl Radical)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Adult
MH  - Chemotactic Factors/*pharmacology
MH  - Free Radicals
MH  - Humans
MH  - Hydroxides/blood
MH  - Hydroxyl Radical
MH  - In Vitro Techniques
MH  - Luminescent Measurements
MH  - Monocytes/*metabolism
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Oxidation-Reduction
MH  - Oxygen/*blood
MH  - Superoxides/blood
MH  - Tetradecanoylphorbol Acetate/pharmacology
EDAT- 1983/12/01 00:00
MHDA- 1983/12/01 00:01
CRDT- 1983/12/01 00:00
PHST- 1983/12/01 00:00 [pubmed]
PHST- 1983/12/01 00:01 [medline]
PHST- 1983/12/01 00:00 [entrez]
AID - 0022-2143(83)90039-2 [pii]
PST - ppublish
SO  - J Lab Clin Med. 1983 Dec;102(6):890-8.