PMID- 6315879
OWN - NLM
STAT- MEDLINE
DCOM- 19840107
LR  - 20190630
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 41
IP  - 6
DP  - 1983 Dec
TI  - Retinal hexokinase: kinetic properties and the effect of cyclic 3',5'-adenosine 
      monophosphate.
PG  - 1694-701
AB  - We measured hexokinase (EC 2.7.1.1) activity in particulate and soluble fractions 
      isolated from bullfrog (Rana catesbeiana) retinas. Seventy-three percent of the 
      hexokinase (HK) activity was associated with the particulate fraction, 27% with 
      the soluble fraction. Both HK fractions could phosphorylate fructose, glucose, 
      2-deoxy-D-glucose, and mannose, but not galactose. The Km for glucose was 0.14 
      mM, for 2-deoxy-D-glucose, 3.6 mM. With glucose as substrate, the Vmax for 
      particulate HK was 125-148 microM retina-1 min-1, for soluble HK, 37 microM 
      retina-1 min-1. Product inhibition of particulate HK activity by glucose 
      6-phosphate was marked, whereas 2-deoxy-D-glucose 6-phosphate did not inhibit the 
      activity. Cyclic AMP stimulated the HK activity of both retinal fractions nearly 
      twofold at concentrations of 0.2-0.8 mM; AMP was much less effective in this 
      regard.
FAU - Liu, S S
AU  - Liu SS
FAU - Witkovsky, P
AU  - Witkovsky P
FAU - Yang, C Y
AU  - Yang CY
LA  - eng
GR  - EY03570/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Glucosephosphates)
RN  - 0 (Hexoses)
RN  - 56-73-5 (Glucose-6-Phosphate)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 2.7.1.1 (Hexokinase)
SB  - IM
MH  - Animals
MH  - Cyclic AMP/*pharmacology
MH  - Glucose-6-Phosphate
MH  - Glucosephosphates/pharmacology
MH  - Hexokinase/*metabolism
MH  - Hexoses/metabolism
MH  - Kinetics
MH  - Rana catesbeiana
MH  - Retina/*enzymology
MH  - Solubility
MH  - Substrate Specificity
MH  - Tissue Distribution
EDAT- 1983/12/01 00:00
MHDA- 1983/12/01 00:01
CRDT- 1983/12/01 00:00
PHST- 1983/12/01 00:00 [pubmed]
PHST- 1983/12/01 00:01 [medline]
PHST- 1983/12/01 00:00 [entrez]
AID - 10.1111/j.1471-4159.1983.tb00882.x [doi]
PST - ppublish
SO  - J Neurochem. 1983 Dec;41(6):1694-701. doi: 10.1111/j.1471-4159.1983.tb00882.x.