PMID- 6322421
OWN - NLM
STAT- MEDLINE
DCOM- 19840406
LR  - 20190714
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 132
IP  - 2
DP  - 1984 Jan 30
TI  - Isolation and characterization of a Mo-MuSV-transformed TB cell line that 
      produces noninfectious MuSV particles with uncleaved gag protein which is 
      processed in the presence of Mo-MuLV.
PG  - 390-400
AB  - A cell line, TBSV7, that produces noninfectious murine sarcoma virus (MuSV) in 
      the absence of helper MuLV was isolated from TB cells infected with the 
      supernatant of MuSV349 cells. These noninfectious MuSV particles with "immature" 
      C-type virus morphology contain a 2.2 X 10(6)-Da genomic RNA and an uncleaved 
      62,000-Da gag precursor protein (Pr62). Neither viral envelope proteins (gp70, 
      p15E, p12E) nor reverse transcriptase were detected in these virus particles. 
      Pr62 was found to be phosphorylated in vivo and it could be phosphorylated in 
      vitro with [gamma-32P]ATP, indicating that protein kinase was packaged in these 
      noninfectious virions. In vitro processing of Pr62 to smaller molecular weight 
      proteins could be achieved by the addition of Mo-MuLV and Nonidet P-40. The 
      initial cleavage products were proteins with molecular weights of 38K (Pr38) and 
      27K (Pr27). Under optimum conditions Pr38 was cleaved to p30 and a protein band 
      migrating with MuLV-p10, while Pr27 was cleaved to a 17,000-Da protein that 
      migrated slower than MuLV-p15 and a protein band migrating with MuLV-p12. 
      Pulse-chase experiments performed on TBSV7 cells superinfected with Mo-MuLV 
      indicated that intracellular processing of Pr62 was much slower than that of 
      Pr65. Cleavage protein products of Pr62 similar in size to the in vitro protein 
      products were also detected in TBSV7 cells superinfected with MuLV.
FAU - Soong, M M
AU  - Soong MM
FAU - Yuen, P H
AU  - Yuen PH
FAU - Wong, P K
AU  - Wong PK
LA  - eng
GR  - CA 19723/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Gene Products, gag)
RN  - 0 (Protein Precursors)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cell Separation
MH  - Cell Transformation, Viral
MH  - Gene Products, gag
MH  - Mice
MH  - Moloney murine leukemia virus/*metabolism
MH  - Phosphorylation
MH  - Protein Precursors/analysis
MH  - Sarcoma Viruses, Murine/*isolation & purification
MH  - Viral Proteins/*metabolism
MH  - Virion/*isolation & purification
EDAT- 1984/01/30 00:00
MHDA- 1984/01/30 00:01
CRDT- 1984/01/30 00:00
PHST- 1984/01/30 00:00 [pubmed]
PHST- 1984/01/30 00:01 [medline]
PHST- 1984/01/30 00:00 [entrez]
AID - 10.1016/0042-6822(84)90044-8 [doi]
PST - ppublish
SO  - Virology. 1984 Jan 30;132(2):390-400. doi: 10.1016/0042-6822(84)90044-8.