PMID- 6328350
OWN - NLM
STAT- MEDLINE
DCOM- 19840712
LR  - 20190726
IS  - 0028-3908 (Print)
IS  - 0028-3908 (Linking)
VI  - 23
IP  - 3
DP  - 1984 Mar
TI  - Binding of buprenorphine to opiate receptors. Regulation by guanyl nucleotides 
      and metal ions.
PG  - 373-5
AB  - The effects of guanosine-5'-triphosphate (GTP), sodium chloride and manganese 
      chloride on the binding of buprenorphine to opiate receptors present in rat brain 
      has been studied. Manganese chloride significantly decreased the affinity of 
      binding of both [3H] buprenorphine and unlabelled buprenorphine to morphine and 
      enkephalin receptors. Guanosine-5'-triphosphate increased the affinity of 
      buprenorphine for morphine sites, but had no effect on binding of buprenorphine 
      to enkephalin or benzomorphan sites, or binding of [3H]buprenorphine. Sodium 
      chloride had no effect on binding of buprenorphine. Control studies indicated 
      similar apparent affinities of buprenorphine for morphine (Ki = 0.30 nM) and 
      enkephalin (Ki = 0.31 nM) sites, and lower affinity for benzomorphan sites (Ki = 
      4.16 nM). No evidence could be obtained for a differential effect of ions or 
      guanosine-5'-triphosphate on binding of buprenorphine to opiate receptor 
      subtypes.
FAU - Villiger, J W
AU  - Villiger JW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Neuropharmacology
JT  - Neuropharmacology
JID - 0236217
RN  - 0 (Morphinans)
RN  - 0 (Receptors, Opioid)
RN  - 40D3SCR4GZ (Buprenorphine)
RN  - 42Z2K6ZL8P (Manganese)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - 9NEZ333N27 (Sodium)
SB  - IM
MH  - Animals
MH  - Binding Sites/drug effects
MH  - Brain/*metabolism
MH  - Buprenorphine/*metabolism
MH  - Guanosine Triphosphate/pharmacology
MH  - In Vitro Techniques
MH  - Manganese/pharmacology
MH  - Morphinans/*metabolism
MH  - Rats
MH  - Receptors, Opioid/*metabolism
MH  - Sodium/metabolism
EDAT- 1984/03/01 00:00
MHDA- 1984/03/01 00:01
CRDT- 1984/03/01 00:00
PHST- 1984/03/01 00:00 [pubmed]
PHST- 1984/03/01 00:01 [medline]
PHST- 1984/03/01 00:00 [entrez]
AID - 10.1016/0028-3908(84)90201-6 [doi]
PST - ppublish
SO  - Neuropharmacology. 1984 Mar;23(3):373-5. doi: 10.1016/0028-3908(84)90201-6.