PMID- 6348525
OWN - NLM
STAT- MEDLINE
DCOM- 19830909
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 113
IP  - 5
DP  - 1983 Aug
TI  - A differential DNA-repair test using mixtures of E. coli K12 strains in liquid 
      suspension and animal-mediated assays.
PG  - 403-15
AB  - The feasibility of performing tests for repairable DNA damage in animal assay 
      procedures was investigated by using repair-proficient and repair-deficient 
      derivatives of E. coli K12 strain 343/113, including mutations in the uvrB, recA, 
      polA and dam genes. To avoid variations in the relative recovery of viable cells 
      from different samples, the strains were further marked with auxotrophic growth 
      requirements, so that mixtures could be treated and the survival of each strain 
      determined individually on media containing the corresponding growth factors. 
      Spot tests were performed with the various strains to re-assess the necessity of 
      using a combination of repair deficiencies, when genotoxic agents of differing 
      mode of action are to be detected. Liquid suspension tests on mixtures of the 
      different strains, furthermore, confirmed that the survival of the individual 
      strains can be determined separately on selective media after treatment with 
      methyl methanesulfonate (MMS) and methyl nitrosourea (MNU). These tests were also 
      used to demonstrate that dimethyl nitrosamine (DMNA) is activated by 
      Aroclor-1254-induced rat-liver S9 fractions to genotoxic products, as measured by 
      the low survival of a recA derivative compared with the repair-proficient 
      wild-type strain. Intrasanguineous host-mediated assays using the present 
      derivatives of E. coli K12/343/113 showed that the various strains, injected 
      simultaneously into mice, could be recovered in amounts sufficient for the 
      individual determination of the relative survival in liver, spleen, lungs, 
      kidneys, pancreas and the blood stream of the host animals. Using a mixture of 
      the repair-proficient parent and the recA derivative inoculated into mice that 
      were subsequently treated with MMS, NMU or DMNA, we found that these chemicals 
      induce a larger decrease in survival in the recA strain as compared with the 
      wild-type in cells recovered from the liver and the spleen. The order of 
      genotoxic potency so determined was DMNA greater than MNU greater than MMS; this 
      is similar to the ranking of the carcinogenicity of these compounds in rodents 
      and probably also reflects the various degrees of DNA alkylation in cells of the 
      livers of the treated animals. The general usefulness of the host-mediated 
      differential DNA repair assay for detecting genotoxic factors in various organs 
      of animals remains to be assessed by using chemical mutagens of different modes 
      of action.
FAU - Mohn, G R
AU  - Mohn GR
FAU - Kerklaan, P R
AU  - Kerklaan PR
FAU - ten Bokkum-Coenradi, W P
AU  - ten Bokkum-Coenradi WP
FAU - ten Hulscher, T E
AU  - ten Hulscher TE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Mutagens)
RN  - 0 (Suspensions)
SB  - IM
MH  - Animals
MH  - DNA Repair/*drug effects
MH  - *Escherichia coli/drug effects
MH  - Mice
MH  - *Mutagenicity Tests/methods
MH  - Mutagens/metabolism/pharmacology
MH  - Rats
MH  - Suspensions
EDAT- 1983/08/01 00:00
MHDA- 1983/08/01 00:01
CRDT- 1983/08/01 00:00
PHST- 1983/08/01 00:00 [pubmed]
PHST- 1983/08/01 00:01 [medline]
PHST- 1983/08/01 00:00 [entrez]
AID - 0165-1161(83)90230-3 [pii]
AID - 10.1016/0165-1161(83)90230-3 [doi]
PST - ppublish
SO  - Mutat Res. 1983 Aug;113(5):403-15. doi: 10.1016/0165-1161(83)90230-3.