PMID- 6348531
OWN - NLM
STAT- MEDLINE
DCOM- 19830920
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 121
IP  - 2
DP  - 1983 Aug
TI  - Cytochrome P-450 factors determining synthesis in strain D7 Saccharomyces 
      cerevisiae. An alternative system to microsomal assay.
PG  - 117-23
AB  - Certain aspects of cytochrome P-450 induction were studied in a diploid strain 
      (D7) of the yeast Saccharomyces cerevisiae in order to obtain cells containing a 
      high level of metabolizing enzymes. The highest level of cytochrome P-450 was 
      reached during the logarithmic growth phase in a 20%-glucose liquid medium. Yeast 
      cells harvested in these conditions were used in the mutagenesis test with 
      dimethyl nitrosamine (DMNA) as a positive control and with styrene (Sty). Both 
      substances gave positive results, whereas Sty never showed any mutagenic activity 
      in the conventional test with stationary growth phase cells and external 
      metabolic activation. The test with cells from the logarithmic growth phase is 
      proposed as a possible alternative to the liver-microsome assay, and its 
      reliability is discussed.
FAU - Del Carratore, R
AU  - Del Carratore R
FAU - Bronzetti, G
AU  - Bronzetti G
FAU - Bauer, C
AU  - Bauer C
FAU - Corsi, C
AU  - Corsi C
FAU - Nieri, R
AU  - Nieri R
FAU - Paolini, M
AU  - Paolini M
FAU - Giagoni, P
AU  - Giagoni P
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Mutagens)
RN  - 0 (Styrenes)
RN  - 44LJ2U959V (Styrene)
RN  - 9035-51-2 (Cytochrome P-450 Enzyme System)
RN  - M43H21IO8R (Dimethylnitrosamine)
SB  - IM
MH  - Animals
MH  - Biotransformation
MH  - Cytochrome P-450 Enzyme System/*metabolism
MH  - Dimethylnitrosamine/toxicity
MH  - Kinetics
MH  - Microsomes, Liver/metabolism
MH  - Mutagenicity Tests
MH  - *Mutagens
MH  - *Mutation
MH  - Saccharomyces cerevisiae/drug effects/growth & development/*metabolism
MH  - Styrene
MH  - Styrenes/toxicity
EDAT- 1983/08/01 00:00
MHDA- 1983/08/01 00:01
CRDT- 1983/08/01 00:00
PHST- 1983/08/01 00:00 [pubmed]
PHST- 1983/08/01 00:01 [medline]
PHST- 1983/08/01 00:00 [entrez]
AID - 0165-7992(83)90109-4 [pii]
AID - 10.1016/0165-7992(83)90109-4 [doi]
PST - ppublish
SO  - Mutat Res. 1983 Aug;121(2):117-23. doi: 10.1016/0165-7992(83)90109-4.