PMID- 6351711
OWN - NLM
STAT- MEDLINE
DCOM- 19831021
LR  - 20061115
IS  - 0003-4193 (Print)
IS  - 0003-4193 (Linking)
VI  - 14
IP  - 2
DP  - 1983
TI  - Virulence factors of enterotoxigenic E. coli studied in the infant mouse model.
PG  - 169-82
AB  - Enterotoxigenic E. coli (ETEC) strain B41 orally inoculated in infant mice at 
      birth induces lethal diarrheoal disease. This model was used to study virulence 
      factors of ETEC strains of bovine and porcine origins. Strains possessing 
      different plasmid-coded virulence factors were obtained either after spontaneous 
      loss of these factors from the original strain or by plasmid transfer to 
      different E. coli. A clone B41A derived from the strain B41 did not produce K99 
      antigen, but still produced heat-stable (ST) toxin. This clone B41A was about as 
      pathogenic as the strain B41 in that it caused death of nearly all animals less 
      than 48 h after inoculation with 10(3) bacteria. Plasmids were transferred from 
      the strain B41 to E. coli C600, to nalidixic acid resistant mutants of strains of 
      serotypes O8, O9, O101 and to an E. coli strain isolated from a suckling mouse 
      intestine. K99+ Ent+ transconjuguant clones were either not all or mildly 
      pathogenic. Two strains of porcine origin K88+ Ent+ were mildly pathogenic for 
      mice. Loss of factor K88 did not reduce virulence. In contrast, loss of both K88 
      and Ent characteristics suppressed virulence. Strain E. coli K12 that had 
      acquired K88 virulence factor possibly with other plasmid-coded characteristics 
      but not the ability to produce ST toxin was non-pathogenic. Hence factors K99 and 
      K88 may not be essential for inducing mortality in the infant mouse model. Other 
      factors may be involved in virulence of the strain B41A. These results were 
      discussed from the viewpoint of pathogenicity for mice and for ETEC natural 
      hosts.
FAU - Bertin, A
AU  - Bertin A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - France
TA  - Ann Rech Vet
JT  - Annales de recherches veterinaires. Annals of veterinary research
JID - 1267230
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Enterotoxins)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Antigens, Bacterial/analysis
MH  - Cattle/microbiology
MH  - Clone Cells
MH  - Diarrhea/*etiology
MH  - Enterotoxins/*biosynthesis
MH  - Escherichia coli/immunology/metabolism/*pathogenicity
MH  - Mice
MH  - Sheep/microbiology
MH  - Virulence
EDAT- 1983/01/01 00:00
MHDA- 1983/01/01 00:01
CRDT- 1983/01/01 00:00
PHST- 1983/01/01 00:00 [pubmed]
PHST- 1983/01/01 00:01 [medline]
PHST- 1983/01/01 00:00 [entrez]
PST - ppublish
SO  - Ann Rech Vet. 1983;14(2):169-82.