PMID- 6355300
OWN - NLM
STAT- MEDLINE
DCOM- 19831217
LR  - 20190723
IS  - 0022-1759 (Print)
IS  - 0022-1759 (Linking)
VI  - 63
IP  - 3
DP  - 1983 Oct 28
TI  - An improved ELISA for anti-native DNA by elimination of interference by 
      anti-histone antibodies.
PG  - 359-66
AB  - We evaluated an enzyme-linked immunosorbent assay (ELISA) for antibodies to 
      native DNA (nDNA) in which protamine was used to link DNA to polystyrene. 
      Elevated anti-nDNA was largely restricted to patients with systemic lupus 
      erythematosus (SLE), and within this group good correlation between ELISA and the 
      ammonium sulfate assay was obtained. However, substantial background 
      immunoglobulin binding to protamine coated wells was commonly observed, and it 
      was necessary to subtract this activity from each anti-DNA determination. Many of 
      the SLE sera also contained anti-histone antibodies, and this antibody activity 
      showed significant correlation with the binding to protamine. In contrast, 
      methylated bovine serum albumin (mBSA) did not bind anti-histone antibodies and 
      provided a substrate for coupling nDNA to polystyrene. This modified ELISA 
      allowed the quantitation of antibodies to native DNA without the simultaneous 
      binding of anti-histone antibodies.
FAU - Rubin, R L
AU  - Rubin RL
FAU - Joslin, F G
AU  - Joslin FG
FAU - Tan, E M
AU  - Tan EM
LA  - eng
GR  - AM32063/AM/NIADDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - J Immunol Methods
JT  - Journal of immunological methods
JID - 1305440
RN  - 0 (Antibodies, Antinuclear)
RN  - 0 (Histones)
RN  - 9007-49-2 (DNA)
RN  - SU46BAM238 (Ammonium Sulfate)
SB  - IM
MH  - Ammonium Sulfate
MH  - Antibodies, Antinuclear/*analysis
MH  - DNA/*immunology
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Histones/*immunology
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Isoelectric Point
MH  - Lupus Erythematosus, Systemic/immunology
EDAT- 1983/10/28 00:00
MHDA- 1983/10/28 00:01
CRDT- 1983/10/28 00:00
PHST- 1983/10/28 00:00 [pubmed]
PHST- 1983/10/28 00:01 [medline]
PHST- 1983/10/28 00:00 [entrez]
AID - S0022-1759(83)80009-X [pii]
AID - 10.1016/s0022-1759(83)80009-x [doi]
PST - ppublish
SO  - J Immunol Methods. 1983 Oct 28;63(3):359-66. doi: 10.1016/s0022-1759(83)80009-x.