PMID- 6363208
OWN - NLM
STAT- MEDLINE
DCOM- 19840228
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 25
IP  - 1
DP  - 1983 Nov
TI  - Molecular cloning of the glyceraldehyde-3-phosphate dehydrogenase genes of 
      Bacillus stearothermophilus and Escherichia coli, and their expression in 
      Escherichia coli.
PG  - 1-7
AB  - Recombinant plasmids derived from pBR322, which carry gap genes coding for the 
      D-glyceraldehyde-3-phosphate dehydrogenases (GAPDH) of Bacillus 
      stearothermophilus and Escherichia coli, have been isolated. The selection was 
      carried out by complementation of an E. coli gapam mutant. Two plasmids 
      containing B. stearothermophilus and E. coli DNA inserts of 4.3 kb and 1.4 kb, 
      respectively, were characterized. Transformation of the E. coli mutant with 
      either of the recombinant plasmids lead to a very high expression of the GAPDH 
      activity. GAPDH produced by the strain containing the B. stearothermophilus gap 
      gene was characterized by immunological cross-reactivity with antiserum raised 
      against pure B. stearothermophilus GAPDH.
FAU - Branlant, G
AU  - Branlant G
FAU - Flesch, G
AU  - Flesch G
FAU - Branlant, C
AU  - Branlant C
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (DNA, Bacterial)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
SB  - IM
MH  - Base Sequence
MH  - Chromosomes, Bacterial
MH  - *Cloning, Molecular
MH  - DNA, Bacterial/biosynthesis
MH  - Escherichia coli/enzymology/*genetics
MH  - Gene Expression Regulation
MH  - *Genes, Bacterial
MH  - Geobacillus stearothermophilus/enzymology/*genetics
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/biosynthesis/*genetics
MH  - Transformation, Genetic
EDAT- 1983/11/01 00:00
MHDA- 1983/11/01 00:01
CRDT- 1983/11/01 00:00
PHST- 1983/11/01 00:00 [pubmed]
PHST- 1983/11/01 00:01 [medline]
PHST- 1983/11/01 00:00 [entrez]
AID - 0378-1119(83)90161-0 [pii]
AID - 10.1016/0378-1119(83)90161-0 [doi]
PST - ppublish
SO  - Gene. 1983 Nov;25(1):1-7. doi: 10.1016/0378-1119(83)90161-0.