PMID- 6373248
OWN - NLM
STAT- MEDLINE
DCOM- 19840709
LR  - 20220511
IS  - 0261-4189 (Print)
IS  - 1460-2075 (Electronic)
IS  - 0261-4189 (Linking)
VI  - 3
IP  - 4
DP  - 1984 Apr
TI  - pBR322 plasmid DNA modified with 2-acetylaminofluorene derivatives: transforming 
      activity and in vitro strand cleavage by the Escherichia coli uvrABC 
      endonuclease.
PG  - 757-60
AB  - Covalently closed circular plasmid DNA was treated with three reactive 
      derivatives of 2-acetylaminofluorene: N-acetoxy-N-2-acetylaminofluorene 
      (N-Aco-AAF), its 7-iodo derivative (N-Aco- AAIF ) and N-hydroxy-N-2-aminofluorene 
      (N-OH-AF), and tested as substrates for the Escherichia coli uvrABC endonuclease 
      and for transformation frequencies on wild-type, uvrA, recA, uvrArecA and polA 
      mutant strains. The uvrABC endonuclease reacted with all three substrates with 
      high efficiency, implicating this enzyme in the repair of DNA containing all 
      three types of adducts. However, only AAF- and AAIF -DNA showed greatly reduced 
      survival on uvrA mutants (five adducts/lethal hit) relative to wild-type (20 
      adducts/lethal hit). AF-DNA survived equally well on uvrA mutant and wild-type 
      cells, and at a much higher level of modification (60 adducts/lethal hit). A 
      mutation in recA had only a minor effect on the survival of either DNA. The polA 
      mutation reduced the survival of the AAF-treated DNA to the same extent as the 
      uvrA mutation (five adducts/lethal hit). Also AF-DNA showed reduced survival on 
      polA mutant cells versus wild-type. However, many more adducts (20/lethal hit) 
      were tolerated than for AAF-DNA, indicating that AF lesions in the template do 
      not efficiently block replication of DNA.
FAU - Fuchs, R P
AU  - Fuchs RP
FAU - Seeberg, E
AU  - Seeberg E
LA  - eng
PT  - Journal Article
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (DNA, Bacterial)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (Fluorenes)
RN  - 43146-76-5 (N-acetoxy-N-2-acetylamino-7-iodofluorene)
RN  - 6098-44-8 (Acetoxyacetylaminofluorene)
RN  - 61M94X4V24 (N-hydroxy-2-aminofluorene)
RN  - 9M98QLJ2DL (2-Acetylaminofluorene)
RN  - EC 3.1.- (Endodeoxyribonucleases)
RN  - EC 3.1.25.- (endodeoxyribonuclease uvrABC)
SB  - IM
MH  - 2-Acetylaminofluorene/*pharmacology
MH  - Acetoxyacetylaminofluorene/analogs & derivatives/pharmacology
MH  - *DNA Repair
MH  - DNA, Bacterial/*metabolism
MH  - Endodeoxyribonucleases/*metabolism
MH  - Escherichia coli/enzymology/*genetics
MH  - *Escherichia coli Proteins
MH  - Fluorenes/pharmacology
MH  - Mutation
MH  - Plasmids
MH  - Substrate Specificity
MH  - Transformation, Bacterial/*drug effects
PMC - PMC557422
EDAT- 1984/04/01 00:00
MHDA- 1984/04/01 00:01
PMCR- 1985/04/01
CRDT- 1984/04/01 00:00
PHST- 1984/04/01 00:00 [pubmed]
PHST- 1984/04/01 00:01 [medline]
PHST- 1984/04/01 00:00 [entrez]
PHST- 1985/04/01 00:00 [pmc-release]
AID - 10.1002/j.1460-2075.1984.tb01880.x [doi]
PST - ppublish
SO  - EMBO J. 1984 Apr;3(4):757-60. doi: 10.1002/j.1460-2075.1984.tb01880.x.