PMID- 6373372
OWN - NLM
STAT- MEDLINE
DCOM- 19840718
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 170
IP  - 2
DP  - 1984 May 21
TI  - Separation of cells labeled with immunospecific iron dextran microspheres using 
      high gradient magnetic chromatography.
PG  - 232-8
AB  - Immunospecific magnetic microspheres, consisting of ferromagnetic iron dextran 
      conjugated to Protein A, were used to specifically label red blood cells (RBC) 
      for cell separation studies using high gradient magnetic chromatography ( HGMC ). 
      When 10(7)-10(8) RBC labeled with Protein A-iron dextran microspheres were 
      applied to a column containing 30 mg stainless steel wire placed in a 7.5 
      kilogauss magnetic field, 96 +/- 2% of the cells were retained in the column. 
      These cells could be eluted by removing the magnetic field and mechanically 
      agitating the column. The retention of labeled cells by HGMC was shown to be 
      dependent on the applied magnetic field and the amount of wire packed into the 
      column. HGMC in conjunction with cell labeling with immunospecific iron dextran 
      microspheres have useful applications for the separation of specific cell types.
FAU - Molday, R S
AU  - Molday RS
FAU - Molday, L L
AU  - Molday LL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Staphylococcal Protein A)
RN  - 9004-66-4 (Iron-Dextran Complex)
SB  - IM
MH  - Cell Separation/*methods
MH  - *Chromatography
MH  - Erythrocytes/*cytology
MH  - Humans
MH  - Immunologic Techniques
MH  - *Iron-Dextran Complex
MH  - *Magnetics
MH  - Microspheres
MH  - Staphylococcal Protein A
EDAT- 1984/05/21 00:00
MHDA- 1984/05/21 00:01
CRDT- 1984/05/21 00:00
PHST- 1984/05/21 00:00 [pubmed]
PHST- 1984/05/21 00:01 [medline]
PHST- 1984/05/21 00:00 [entrez]
AID - 0014-5793(84)81319-8 [pii]
AID - 10.1016/0014-5793(84)81319-8 [doi]
PST - ppublish
SO  - FEBS Lett. 1984 May 21;170(2):232-8. doi: 10.1016/0014-5793(84)81319-8.