PMID- 6383014
OWN - NLM
STAT- MEDLINE
DCOM- 19841025
LR  - 20190511
IS  - 0002-9173 (Print)
IS  - 0002-9173 (Linking)
VI  - 82
IP  - 4
DP  - 1984 Oct
TI  - Reappraisal of the specificity of the Crithidia luciliae assay for nDNA 
      antibodies: evidence for histone antibody kinetoplast binding.
PG  - 448-52
AB  - Five different high-titer histone antibody-containing sera were assayed by the 
      Crithidia luciliae indirect immunofluorescence (CLIF) technic. Three of these 
      sera produced kinetoplast binding at titers of 1/40 to 1/80. The kinetoplast 
      binding activity was abolished by HCl acid pretreatment of the Crithidia 
      substrate, suggesting that the kinetoplast binding activity was not due to 
      antibodies against native DNA (nDNA). Histone antibodies were purified from two 
      of the three positive sera by affinity chromatography utilizing purified 
      preparations of histone. Both purified antibody preparations also had 
      kinetoplast-binding activity, confirming that the Crithidia kinetoplast contains 
      histone-like proteins. Therefore, Crithidia luciliae (CL) kinetoplast binding 
      activity does not necessarily indicate the presence of anti-nDNA antibodies. 
      Routinely pretreating the CL substrate with 0.1 N HCl would eliminate the 
      possibility of histone antibody kinetoplast binding in the CLIF assay. Whether 
      such pretreatment would alter the binding of anti-NDNA to the kinetoplast remains 
      to be determined.
FAU - Deng, J S
AU  - Deng JS
FAU - Rubin, R L
AU  - Rubin RL
FAU - Lipscomb, M F
AU  - Lipscomb MF
FAU - Sontheimer, R D
AU  - Sontheimer RD
FAU - Gilliam, J N
AU  - Gilliam JN
LA  - eng
GR  - AI 17363/AI/NIAID NIH HHS/United States
GR  - AM 00730/AM/NIADDK NIH HHS/United States
GR  - AM 19101/AM/NIADDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 0 (Antibodies)
RN  - 0 (Antibodies, Antinuclear)
RN  - 0 (Histones)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Antibodies/*analysis
MH  - Antibodies, Antinuclear/*analysis
MH  - Crithidia
MH  - DNA/*immunology
MH  - Fluorescent Antibody Technique
MH  - Histones/*immunology
MH  - Humans
MH  - Lupus Erythematosus, Systemic/immunology
MH  - Methods
EDAT- 1984/10/01 00:00
MHDA- 1984/10/01 00:01
CRDT- 1984/10/01 00:00
PHST- 1984/10/01 00:00 [pubmed]
PHST- 1984/10/01 00:01 [medline]
PHST- 1984/10/01 00:00 [entrez]
AID - 10.1093/ajcp/82.4.448 [doi]
PST - ppublish
SO  - Am J Clin Pathol. 1984 Oct;82(4):448-52. doi: 10.1093/ajcp/82.4.448.