PMID- 6389977
OWN - NLM
STAT- MEDLINE
DCOM- 19850117
LR  - 20131121
IS  - 0023-6837 (Print)
IS  - 0023-6837 (Linking)
VI  - 51
IP  - 6
DP  - 1984 Dec
TI  - Inhibition of glomerular visceral epithelial cell endocytosis during nephrosis 
      induced by puromycin aminonucleoside.
PG  - 690-6
AB  - Puromycin aminonucleoside (PA), injected intravenously into rats in a single 
      dose, causes proteinuria and ultrastructural pathology reminiscent of human 
      lipoid nephrosis (puromycin aminonucleoside nephrosis (PAN]. Glomerular 
      epithelial cell (GEC) endocytosis was studied in this model and in rats with 
      protein-overload proteinuria using ultrastructural morphometry. Disappearance 
      curves were constructed for protamine-heparin aggregates (PHA), which localized 
      in the subepithelial region of the glomerular basal lamina following intravenous 
      injection of protamine followed by heparin. Five groups were studied: (a) 
      preproteinuric PAN, 4 days after PA (mean 4.5 +/- 1.5 mg of urinary protein/24 
      hours); (b) proteinuric PAN, 10 days after PA (mean 128 +/- 9.6 mg/24 hours); (c) 
      recovery from PAN (mean 12.5 +/- 1.5 mg/24 hours); (d) protein-overload 
      proteinuria, induced by injecting albumin intraperitoneally (mean 211 +/- 15.9 
      mg/24 hours); and (e) saline-injected controls (mean 1.2 +/- 0.2 mg/24 hours). 
      Only the proteinuric PAN animals (group 2) had altered GEC endocytosis with a PHA 
      half-disappearance time different from the group 5 saline controls (143.2 versus 
      72.6 minutes, p less than 0.05). The half-disappearance times in groups 1, 3, and 
      4 were 74.6, 80.7 and 86.5 minutes, respectively. Altered GEC function was 
      further characterized by comparing PHA disappearance with the abundance of 
      albumin-filled vacuoles in the GEC. Prolongation of PHA disappearance in group 2 
      correlated with the virtual absence of vacuoles; they were abundant in 
      nonproteinuric phases of PAN and in animals with overload proteinuria. We 
      conclude (a) GEC endocytosis is reduced only during the proteinuric phase of PAN, 
      (b) GEC endocytosis is active during the preproteinuric phase of PAN and is a 
      factor that may account for the absence of protein in the urine despite abnormal 
      GBM permeability, (c) decreased GEC endocytosis during proteinuric PAN reflects 
      abnormal cell metabolism due to PA and is not simply a consequence of 
      albuminuria, as overload proteinuria did not produce diminished PHA or albumin 
      uptake.
FAU - Schwartz, M M
AU  - Schwartz MM
FAU - Sharon, Z
AU  - Sharon Z
FAU - Pauli, B U
AU  - Pauli BU
FAU - Lewis, E J
AU  - Lewis EJ
LA  - eng
GR  - I RO1AM 21536/AM/NIADDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Lab Invest
JT  - Laboratory investigation; a journal of technical methods and pathology
JID - 0376617
RN  - 0 (Protamines)
RN  - 0 (protamine heparin aggregate)
RN  - 4A6ZS6Q2CL (Puromycin)
RN  - 58-60-6 (Puromycin Aminonucleoside)
RN  - 9005-49-6 (Heparin)
SB  - IM
MH  - Animals
MH  - *Endocytosis
MH  - Epithelium/physiopathology
MH  - Fluorescent Antibody Technique
MH  - Heparin
MH  - Kidney Glomerulus/*physiopathology/ultrastructure
MH  - Kinetics
MH  - Male
MH  - Microscopy, Electron
MH  - Nephrosis, Lipoid/chemically induced/pathology/*physiopathology
MH  - Protamines
MH  - Proteinuria
MH  - *Puromycin/analogs & derivatives
MH  - *Puromycin Aminonucleoside
MH  - Rats
MH  - Rats, Inbred Strains
EDAT- 1984/12/01 00:00
MHDA- 1984/12/01 00:01
CRDT- 1984/12/01 00:00
PHST- 1984/12/01 00:00 [pubmed]
PHST- 1984/12/01 00:01 [medline]
PHST- 1984/12/01 00:00 [entrez]
PST - ppublish
SO  - Lab Invest. 1984 Dec;51(6):690-6.