PMID- 6392220
OWN - NLM
STAT- MEDLINE
DCOM- 19850221
LR  - 20190904
IS  - 0198-8859 (Print)
IS  - 0198-8859 (Linking)
VI  - 11
IP  - 4
DP  - 1984 Dec
TI  - Detection of HLA restricted anti-minor histocompatibility antigen(s) reactive 
      cells from skin GVHD lesions.
PG  - 249-57
AB  - Recipients of marrow grafts from HLA-identical, (including MLC non-reactive) 
      sibling donors have a 40-50% incidence of acute graft vs. host disease (GVHD); 
      the involvement of non-HLA linked, minor histocompatibility antigens (miHA) has 
      been implicated. One of the target tissues of GVHD is the skin where 
      morphological analysis of biopsy specimens is often used to support this 
      diagnosis. We have obtained skin biopsy specimens from the site of GVHD lesions, 
      grown the cells in the presence of T cell growth factor (TCGF) and feeder cells, 
      and tested these cultured cells in the primed lymphocyte test (PLT) and cell 
      mediated lympholysis (CML) assay. Five of six cell cultures tested demonstrated 
      secondary proliferative but not cytolytic reactivity; cells from one culture 
      demonstrated both reactivities. The cell culture populations generated are 
      presumably directed against non-HLA antigens, i.e., miHA whose expression is 
      restricted by an HLA antigen of the recipient. The data are consistent with the 
      suggestion that in the majority of these cultures, the restriction element may be 
      a determinant encoded within the HLA-D region: DQ, DR, and possibly DP based on 
      panel testing. Although the number of cases is small, these preliminary data 
      demonstrate the feasibility of this type of culturing system and also suggest 
      that the cellular immunological events leading to the manifestation of skin GVHD 
      lesions may be predominantly a delayed type hypersensitivity reaction.
FAU - Reinsmoen, N L
AU  - Reinsmoen NL
FAU - Kersey, J H
AU  - Kersey JH
FAU - Bach, F H
AU  - Bach FH
LA  - eng
GR  - AI 18326/AI/NIAID NIH HHS/United States
GR  - AI 19007/AI/NIAID NIH HHS/United States
GR  - R01 AI 17687/AI/NIAID NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (HLA Antigens)
RN  - 0 (Histocompatibility Antigens Class II)
SB  - IM
MH  - Adult
MH  - Bone Marrow Transplantation
MH  - Cells, Cultured
MH  - Child
MH  - Cytotoxicity, Immunologic
MH  - Graft vs Host Disease/*immunology/pathology
MH  - HLA Antigens/*immunology
MH  - Histocompatibility Antigens Class II/immunology
MH  - Humans
MH  - Lymphocyte Activation
MH  - Skin/*immunology/pathology
MH  - T-Lymphocytes/immunology
EDAT- 1984/12/01 00:00
MHDA- 1984/12/01 00:01
CRDT- 1984/12/01 00:00
PHST- 1984/12/01 00:00 [pubmed]
PHST- 1984/12/01 00:01 [medline]
PHST- 1984/12/01 00:00 [entrez]
AID - 0198-8859(84)90064-8 [pii]
AID - 10.1016/0198-8859(84)90064-8 [doi]
PST - ppublish
SO  - Hum Immunol. 1984 Dec;11(4):249-57. doi: 10.1016/0198-8859(84)90064-8.