PMID- 6395954
OWN - NLM
STAT- MEDLINE
DCOM- 19850412
LR  - 20180215
IS  - 0008-6312 (Print)
IS  - 0008-6312 (Linking)
VI  - 71
IP  - 6
DP  - 1984
TI  - Chronic Chagas' myocardiopathy. Demonstration of in vivo bound immunoglobulins in 
      heart structures by the immunoperoxidase technique.
PG  - 297-306
AB  - Chagas' disease (CHDis) has been described as the commonest form of chronic 
      myocarditis. Although autoimmune phenomena involving the myocardium have been 
      suggested in both human and experimental CHDis, no immunoperoxidase techniques 
      have been used for the detection of immunological deposits in chronic Chagas' 
      myocardiopathy (ChrCHM). The goal of this paper was to show that there is a 
      coincidence between the immunoperoxidase and the immunofluorescence techniques in 
      evidencing autologous deposits in ChrCHM. 4 patients suffering from ChrCHM were 
      selected; their ages ranged from 39 to 52 years (means: 48.5 years). The Shultze 
      and Caves endocavitary bioptome via the right internal jugular vein was used to 
      obtain 3 to 4 right endomyocardial specimens measuring 1-2 mm each. Direct 
      immunofluorescence examination, immunoperoxidase technique (PAP), and routine 
      histological procedure were performed in all cases. Agreement between 
      immunofluorescence and immunoperoxidase techniques was present in all chagasic 
      specimens studied. Patients suffering from ChrCHM showed deposits of IgG, IgA, 
      IgM, and C3 at the interstitial area, capillaries and endocardium and/or 
      myocardial fiber surfaces when serum EVI antibody was positive. Serum EVI 
      antibody was found to be positive in 3 out of 4 patients. The presence of the 
      complement together with immunoglobulin may suggest an immune reaction (in vivo 
      bound immunoglobulins in heart structures) and not just a passive deposition 
      secondary to myocardial damage. As EVI antibody is present to a significant 
      extent in CHDis, a reliable and simple technique like immunoperoxidase may be 
      helpful to clarify the role of these antibodies in the pathogenesis of CHDis.
FAU - Molina, H A
AU  - Molina HA
FAU - Milei, J
AU  - Milei J
FAU - Storino, R
AU  - Storino R
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Cardiology
JT  - Cardiology
JID - 1266406
RN  - 0 (Complement C3)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulin M)
RN  - 0 (Immunoglobulins)
SB  - IM
MH  - Adult
MH  - Chagas Cardiomyopathy/*immunology
MH  - Chronic Disease
MH  - Complement C3/analysis
MH  - Endocardium/immunology
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Immunoglobulin G/analysis
MH  - Immunoglobulin M/analysis
MH  - Immunoglobulins/*analysis
MH  - Male
MH  - Middle Aged
MH  - Myocardium/*immunology
EDAT- 1984/01/01 00:00
MHDA- 1984/01/01 00:01
CRDT- 1984/01/01 00:00
PHST- 1984/01/01 00:00 [pubmed]
PHST- 1984/01/01 00:01 [medline]
PHST- 1984/01/01 00:00 [entrez]
AID - 10.1159/000173683 [doi]
PST - ppublish
SO  - Cardiology. 1984;71(6):297-306. doi: 10.1159/000173683.