PMID- 6398008
OWN - NLM
STAT- MEDLINE
DCOM- 19850501
LR  - 20211216
IS  - 0300-5410 (Print)
IS  - 0300-5410 (Linking)
VI  - 135B
IP  - 3
DP  - 1984 Nov-Dec
TI  - [Method for the detection and determination of heat-labile Escherichia coli 
      enterotoxin by an immunoenzyme technic on a new polystyrene support adapted for a 
      test kit].
PG  - 297-310
AB  - An ELISA method on microtitration plates to detect and assay Escherichia coli 
      heat-labile enterotoxin (LT) is described. This technique is rapid and simple to 
      perform in any laboratory. It allows detection of the presence of LT with the 
      naked eye within 10 h in a 12-h E. coli culture supernatant. The reaction is 
      based on immunological cross-reaction between LT and the Vibrio cholerae toxin 
      (CT). In place of traditional microtitration plates coated with ganglioside Gm1, 
      we propose a new polystyrene support coated with purified anti-CT antibodies. 
      This coated support has been conditioned in a kit to be used in laboratories in 
      bush dispensaries of endemic areas. It was tested with 40 enterotoxigenic (LT+) 
      strains isolated from stools of diarrhoeal children and with 14 LT- strains. All 
      supernatants LT+ and LT- were found positive and negative, respectively, with the 
      ELISA method and with the new polystyrene support. Field tests (in Wallis, Futuna 
      et Vanuatu) with the new kit and standard method were satisfactory.
FAU - Germani, Y
AU  - Germani Y
FAU - Begaud, E
AU  - Begaud E
FAU - Dassy, B
AU  - Dassy B
FAU - Legonidec, G
AU  - Legonidec G
FAU - Guesdon, J L
AU  - Guesdon JL
LA  - fre
PT  - Journal Article
TT  - Methode de detection et de dosage de l'enterotoxine thermolabile de Escherichia 
      coli par une technique immunoenzymatique sur un nouveau support de polystyrene 
      adapte en trousse autonome.
PL  - France
TA  - Ann Microbiol (Paris)
JT  - Annales de microbiologie
JID - 0354704
RN  - 0 (Bacterial Toxins)
RN  - 0 (Enterotoxins)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (Reagent Kits, Diagnostic)
RN  - 9012-63-9 (Cholera Toxin)
RN  - D9K3SN2LNY (heat-labile enterotoxin, E coli)
SB  - IM
MH  - Bacterial Toxins/*analysis/biosynthesis/immunology
MH  - Cholera Toxin/immunology
MH  - Enterotoxins/*analysis/biosynthesis/immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - *Escherichia coli/metabolism
MH  - *Escherichia coli Proteins
MH  - Reagent Kits, Diagnostic
EDAT- 1984/11/01 00:00
MHDA- 1984/11/01 00:01
CRDT- 1984/11/01 00:00
PHST- 1984/11/01 00:00 [pubmed]
PHST- 1984/11/01 00:01 [medline]
PHST- 1984/11/01 00:00 [entrez]
PST - ppublish
SO  - Ann Microbiol (Paris). 1984 Nov-Dec;135B(3):297-310.