PMID- 6398186
OWN - NLM
STAT- MEDLINE
DCOM- 19850607
LR  - 20191023
IS  - 0147-9571 (Print)
IS  - 0147-9571 (Linking)
VI  - 7
IP  - 3-4
DP  - 1984
TI  - Immunological properties of porcine enterotoxigenic Escherichia coli heat-stable 
      (ST) enterotoxins.
PG  - 157-64
AB  - Cross-linking semipurified and purified heat-stable enterotoxin (2023 ST) to 
      carrier proteins with glutaraldehyde pigs and rabbits were immunized. With ST 
      antisera thus raised, a relatively simple, two-step method for isolation of ST is 
      described. The method involved immunoadsorbent column chromatography procedure, 
      and elution of the retained material with 6 M urea at pH 3.0 yielded purified ST 
      with enterotoxin activity, controlled in suckling mouse test. ST purity was 
      checked with a special staining technique in PAGE, anionic-exchange 
      chromatography and immunodiffusion.
FAU - Pesti, L
AU  - Pesti L
FAU - Lukacs, K
AU  - Lukacs K
LA  - eng
PT  - Journal Article
PL  - England
TA  - Comp Immunol Microbiol Infect Dis
JT  - Comparative immunology, microbiology and infectious diseases
JID - 7808924
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Bacterial Toxins)
RN  - 0 (Enterotoxins)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (heat stable toxin (E coli))
SB  - IM
MH  - Animals
MH  - Antibodies, Bacterial/*analysis
MH  - Antibody Specificity
MH  - *Bacterial Toxins
MH  - Enterotoxins/*immunology
MH  - Escherichia coli/*immunology
MH  - Escherichia coli Proteins
MH  - Immunosorbent Techniques
MH  - Rabbits
MH  - Swine
EDAT- 1984/01/01 00:00
MHDA- 1984/01/01 00:01
CRDT- 1984/01/01 00:00
PHST- 1984/01/01 00:00 [pubmed]
PHST- 1984/01/01 00:01 [medline]
PHST- 1984/01/01 00:00 [entrez]
AID - 0147-9571(84)90021-3 [pii]
AID - 10.1016/0147-9571(84)90021-3 [doi]
PST - ppublish
SO  - Comp Immunol Microbiol Infect Dis. 1984;7(3-4):157-64. doi: 
      10.1016/0147-9571(84)90021-3.