PMID- 6400830
OWN - NLM
STAT- MEDLINE
DCOM- 19900314
LR  - 20131121
IS  - 0739-1102 (Print)
IS  - 0739-1102 (Linking)
VI  - 1
IP  - 6
DP  - 1984 Jun
TI  - Denaturation of nucleic acids induced by intercalating agents. Biochemical and 
      biophysical properties of acridine orange-DNA complexes.
PG  - 1485-99
AB  - At high binding densities acridine orange (AO) forms complexes with ds DNA which 
      are insoluble in aqueous media. These complexes are characterized by high red- 
      and minimal green-luminescence, 1:1 (dye/P) stoichiometry and resemble complexes 
      of AO with ss nucleic acids. Formation of these complexes can be conveniently 
      monitored by light scatter measurements. Light scattering properties of these 
      complexes are believed to result from the condensation of nucleic acids induced 
      by the cationic, intercalating ligands. The spectral and thermodynamic data 
      provide evidence that AO (and other intercalating agents) induces denaturation of 
      ds nucleic acids; the driving force of the denaturation is high affinity and 
      cooperativity of binding of these ligands to ss nucleic acids. The denaturing 
      effects of AO, adriamycin and ellipticine were confirmed by biochemical studies 
      on accessibility of DNA bases (in complexes with these ligands) to the external 
      probes. The denaturing properties of AO vary depending on the primary structure 
      (sugar- and base-composition) of nucleic acids.
FAU - Kapuscinski, J
AU  - Kapuscinski J
AD  - Memorial Sloan-Kettering Cancer Center, Walker Laboratory, Rye, New York 10580.
FAU - Darzynkiewicz, Z
AU  - Darzynkiewicz Z
LA  - eng
GR  - CA23296/CA/NCI NIH HHS/United States
GR  - CA28704/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Biomol Struct Dyn
JT  - Journal of biomolecular structure & dynamics
JID - 8404176
RN  - 0 (Intercalating Agents)
RN  - 9007-49-2 (DNA)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - *Acridine Orange
MH  - Binding Sites
MH  - *DNA
MH  - Intercalating Agents
MH  - Light
MH  - Nucleic Acid Denaturation
MH  - Scattering, Radiation
EDAT- 1984/06/01 00:00
MHDA- 1984/06/01 00:01
CRDT- 1984/06/01 00:00
PHST- 1984/06/01 00:00 [pubmed]
PHST- 1984/06/01 00:01 [medline]
PHST- 1984/06/01 00:00 [entrez]
AID - 10.1080/07391102.1984.10507532 [doi]
PST - ppublish
SO  - J Biomol Struct Dyn. 1984 Jun;1(6):1485-99. doi: 10.1080/07391102.1984.10507532.