PMID- 6402030
OWN - NLM
STAT- MEDLINE
DCOM- 19830415
LR  - 20190609
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 750
IP  - 1
DP  - 1983 Jan 7
TI  - Identification of lipoxygenase products from arachidonic acid metabolism in 
      stimulated murine eosinophils.
PG  - 78-90
AB  - The presence of arachidonic acid lipoxygenase pathways in murine eosinophils was 
      demonstrated by the isolation and identification of several lipoxygenase products 
      from incubations of these cells. The most abundant arachidonate metabolite from 
      murine eosinophils stimulated with ionophore A23187 and exogenous arachidonic 
      acid was 12-S-hydroxyeicosatetraenoic acid (12-S-HETE), and the next most 
      abundant was 15-HETE. Two families of leukotrienes were also recovered from these 
      incubations. One family comprised the hydrolysis products of leukotriene A4, and 
      the other included products derived from the 14,15-oxido analog of leukotriene A4 
      (14,15-leukotriene A4). Two double oxygenation products of arachidonate were also 
      identified. These compounds were a 5,15-dihydroxyeicosatetraenoic acid 
      (5,15-diHETE) and a 5,12-dihydroxyeicosatetraenoic acid (5,12-diHETE). Eosinophil 
      stimulation promoter is a murine lymphokine which enhances the migration of 
      eosinophils. When murine eosinophils were incubated with eosinophil stimulation 
      promoter in concentrations sufficient to produce a migration response, a 2-3-fold 
      increase in the production of 12-HETE was observed compared to unstimulated 
      cells. Coupled with the recent demonstration that arachidonic acid lipoxygenase 
      inhibitors suppress the migration response to eosinophil stimulation promoter and 
      that 12-HETE induces a migration response, this observation provides further 
      evidence in support of the hypothesis that eosinophil stimulation promoter 
      stimulation of eosinophils results in the generation of lipoxygenase products 
      which modulate the migratory activity of the cells.
FAU - Turk, J
AU  - Turk J
FAU - Rand, T H
AU  - Rand TH
FAU - Maas, R L
AU  - Maas RL
FAU - Lawson, J A
AU  - Lawson JA
FAU - Brash, A R
AU  - Brash AR
FAU - Roberts, L J 2nd
AU  - Roberts LJ 2nd
FAU - Colley, D G
AU  - Colley DG
FAU - Oates, J A
AU  - Oates JA
LA  - eng
GR  - 2T32 GM07397/GM/NIGMS NIH HHS/United States
GR  - AI-11289/AI/NIAID NIH HHS/United States
GR  - GM-15431/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Arachidonic Acids)
RN  - 0 (Prostaglandins F)
RN  - 27YG812J1I (Arachidonic Acid)
RN  - B7IN85G1HY (Dinoprost)
RN  - EC 1.13.11.- (Arachidonate Lipoxygenases)
RN  - EC 1.13.11.12 (Lipoxygenase)
SB  - IM
MH  - Animals
MH  - Arachidonate Lipoxygenases
MH  - Arachidonic Acid
MH  - Arachidonic Acids/*blood
MH  - Dinoprost
MH  - Eosinophils/*metabolism
MH  - Kinetics
MH  - Lipoxygenase/*blood
MH  - Mass Spectrometry
MH  - Mice
MH  - Prostaglandins F/blood
EDAT- 1983/01/07 00:00
MHDA- 1983/01/07 00:01
CRDT- 1983/01/07 00:00
PHST- 1983/01/07 00:00 [pubmed]
PHST- 1983/01/07 00:01 [medline]
PHST- 1983/01/07 00:00 [entrez]
AID - 0005-2760(83)90206-0 [pii]
AID - 10.1016/0005-2760(83)90206-0 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1983 Jan 7;750(1):78-90. doi: 10.1016/0005-2760(83)90206-0.