PMID- 6404983
OWN - NLM
STAT- MEDLINE
DCOM- 19830623
LR  - 20220408
IS  - 0022-1554 (Print)
IS  - 0022-1554 (Linking)
VI  - 31
IP  - 6
DP  - 1983 Jun
TI  - A flow cytometric assay of neutrophil degranulation.
PG  - 737-44
AB  - A quantitative assay of neutrophil degranulation was developed using flow 
      cytometry. Dog neutrophils were purified to greater than 95% purity and viability 
      by isopyknic density centrifugation in an isosmotic medium. These cells 
      concentrated the fluorochrome acridine orange (AO) in their azurophilic granules, 
      but not in specific granules. Also contained in the azurophilic granules are 
      elastase, myeloperoxidase, and approximately 50% of the lysozyme activity. The 
      fluorochrome was released concomitantly with elastase activity, as shown by flow 
      cytometry, fluorescence microscopy, and biochemical assay in response to the 
      ionophore A23187. By flow cytometry, unstimulated cells are distributed in a 
      single broad peak of high fluorescence intensity. With increasing concentrations 
      of A23187 (0.48-4.80 microM), a greater proportion of the cells shifted to a 
      single peak of low fluorescence intensity. Few cells with intermediate 
      fluorescence were observed. These analyses revealed that the neutrophils 
      degranulated in a quantal, all-or-none response.
FAU - Abrams, W R
AU  - Abrams WR
FAU - Diamond, L W
AU  - Diamond LW
FAU - Kane, A B
AU  - Kane AB
LA  - eng
GR  - 5PO1HL20994/HL/NHLBI NIH HHS/United States
GR  - SO7RR054-17/RR/NCRR NIH HHS/United States
GR  - SO7RR05505-16/RR/NCRR NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
RN  - 37H9VM9WZL (Calcimycin)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - Calcimycin/pharmacology
MH  - Dogs
MH  - Flow Cytometry/methods
MH  - Fluorescence
MH  - Male
MH  - Neutrophils/*immunology
MH  - Phagocytosis/drug effects
EDAT- 1983/06/01 00:00
MHDA- 1983/06/01 00:01
CRDT- 1983/06/01 00:00
PHST- 1983/06/01 00:00 [pubmed]
PHST- 1983/06/01 00:01 [medline]
PHST- 1983/06/01 00:00 [entrez]
AID - 10.1177/31.6.6404983 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 1983 Jun;31(6):737-44. doi: 10.1177/31.6.6404983.