PMID- 6405454
OWN - NLM
STAT- MEDLINE
DCOM- 19830610
LR  - 20190825
IS  - 0090-6980 (Print)
IS  - 0090-6980 (Linking)
VI  - 25
IP  - 1
DP  - 1983 Jan
TI  - Resolution by DEAE-cellulose chromatography of the enzymatic steps in the 
      transformation of arachidonic acid into 8, 11, 12- and 10, 11, 
      12-trihydroxy-eicosatrienoic acid by the rat lung.
PG  - 79-84
AB  - The 30-50% ammonium sulfate fraction of the high speed supernatant (100,000 xg) 
      of a rat lung homogenate is capable of catalysing the conversion of arachidonic 
      acid into 8,11,12- and 10,11, 12-trihydroxyeicosatrienoic acids. This enzyme 
      preparation was resolved through DEAE cellulose chromatography into three stages 
      which were assayed with precursors specific for each stage. Thus in the first 
      stage arachidonic acid is converted by 12-lipoxygenase into 
      12-hydroperoxy-5,8,10,14-eicosatetraenoic acid (12-HPETE) detected as the 
      corresponding 12-hydroxy product (12-HETE). 12-HPETE in turn is converted into 
      8-hydroxy-11,12-epoxy-5,9,14-eicosatrienoic acid and 
      10-hydroxy-11,12-epoxy-5,8,14-eicosatrienoic acid. These epoxides are in turn 
      selectively converted through an epoxide hydrase into the respective triols. 
      While the first and third stages were carried out by distinct fractions from the 
      DEAE columns, the second i.e. conversion of 12-HPETE into epoxides, was detected 
      in all fractions as was the reduction of 12-HPETE into 12-HETE.
FAU - Pace-Asciak, C R
AU  - Pace-Asciak CR
FAU - Mizuno, K
AU  - Mizuno K
FAU - Yamamoto, S
AU  - Yamamoto S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Prostaglandins
JT  - Prostaglandins
JID - 0320271
RN  - 0 (Arachidonic Acids)
RN  - 0 (Fatty Acids, Unsaturated)
RN  - 0 (Leukotrienes)
RN  - 27YG812J1I (Arachidonic Acid)
RN  - 59985-28-3 (12-Hydroxy-5,8,10,14-eicosatetraenoic Acid)
RN  - 67675-13-2 (12-HPETE)
RN  - 68860-46-8 (8,11,12-trihydroxy-5,9,14-eicosatrienoic acid)
RN  - 85589-25-9 (10,11,12-trihydroxyeicosatrienoic acid)
RN  - EC 1.13.11.12 (Lipoxygenase)
RN  - EC 3.3.2.- (Epoxide Hydrolases)
RN  - FC398RK06S (8,11,14-Eicosatrienoic Acid)
SB  - IM
MH  - 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid
MH  - 8,11,14-Eicosatrienoic Acid/analogs & derivatives/*biosynthesis
MH  - Animals
MH  - Arachidonic Acid
MH  - Arachidonic Acids/*metabolism
MH  - Biotransformation
MH  - Chromatography, DEAE-Cellulose/methods
MH  - Epoxide Hydrolases/metabolism
MH  - Fatty Acids, Unsaturated/*biosynthesis
MH  - *Leukotrienes
MH  - Lipoxygenase/metabolism
MH  - Lung/*metabolism
MH  - Rats
EDAT- 1983/01/01 00:00
MHDA- 1983/01/01 00:01
CRDT- 1983/01/01 00:00
PHST- 1983/01/01 00:00 [pubmed]
PHST- 1983/01/01 00:01 [medline]
PHST- 1983/01/01 00:00 [entrez]
AID - 0090-6980(83)90137-5 [pii]
AID - 10.1016/0090-6980(83)90137-5 [doi]
PST - ppublish
SO  - Prostaglandins. 1983 Jan;25(1):79-84. doi: 10.1016/0090-6980(83)90137-5.