PMID- 6415114
OWN - NLM
STAT- MEDLINE
DCOM- 19831217
LR  - 20181113
IS  - 0021-9738 (Print)
IS  - 0021-9738 (Linking)
VI  - 72
IP  - 5
DP  - 1983 Nov
TI  - Effect of muscle glycogen depletion on in vivo insulin action in man.
PG  - 1605-10
AB  - In rats, muscle glycogen depletion has been associated with increased insulin 
      action. Whether this also occurs in man has not been reported. After 4 d rest, 13 
      males (E Group) had a percutaneous muscle biopsy of the vastus lateralis muscle 
      followed by a euglycemic clamp at plasma insulin congruent to 100 microU/ml and 
      congruent to 1,900 microU/ml, with simultaneous indirect calorimetry. This was 
      repeated 1 wk later, but after glycogen-depleting exercise the night before the 
      euglycemic clamp. Seven subjects underwent the same protocol but were also re-fed 
      100 g carbohydrate (CHO) after the exercise (EF group). In both groups, the mean 
      muscle glycogen content was approximately 40% lower (P less than 0.01) after 
      exercise compared with the muscle glycogen content measured after rest. In the E 
      group, the mean muscle glycogen synthase activity (percent independent of 
      glucose-6-phosphate) increased threefold (P less than 0.001) after exercise, but 
      increased only twofold in the EF group (P less than 0.02 between groups). In both 
      groups, the mean basal and insulin-stimulated CHO oxidation rates were lower in 
      the post-exercise, glycogen-depleted condition compared with the rested, 
      glycogen-replete condition. The mean insulin-stimulated CHO storage rate 
      increased significantly in the E group after exercise but not in the EF group. In 
      the E group, the total insulin-stimulated CHO disposal rate (M) was 17 (P less 
      than 0.04) and 10% (P less than 0.03) higher after exercise during the low and 
      high dose insulin infusion, respectively. No significant changes in M were 
      observed in the EF group. For all subjects, after rest and exercise, the M 
      correlated with the CHO storage rates during the low (r = 0.80, P less than 
      0.001) and high dose (r = 0.77, P less than 0.001) insulin infusions. After 
      exercise, the muscle glycogen synthase activity correlated with the CHO storage 
      rate (r = 0.73, P less than 0.002; r = 0.75, P less than 0.002) during the low 
      and high dose insulin infusions, respectively, and also with M (r = 0.64, P less 
      than 0.008; r = 0.57; P less than 0.02).
FAU - Bogardus, C
AU  - Bogardus C
FAU - Thuillez, P
AU  - Thuillez P
FAU - Ravussin, E
AU  - Ravussin E
FAU - Vasquez, B
AU  - Vasquez B
FAU - Narimiga, M
AU  - Narimiga M
FAU - Azhar, S
AU  - Azhar S
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Clin Invest
JT  - The Journal of clinical investigation
JID - 7802877
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Insulin)
RN  - 9005-79-2 (Glycogen)
RN  - EC 2.4.1.11 (Glycogen Synthase)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Adult
MH  - Dietary Carbohydrates/metabolism
MH  - Glucose
MH  - Glycogen/*metabolism
MH  - Glycogen Synthase/metabolism
MH  - Humans
MH  - Insulin/*pharmacology
MH  - Male
MH  - Muscles/*metabolism
MH  - *Physical Exertion
PMC - PMC370448
OID - NASA: 84033191
EDAT- 1983/11/01 00:00
MHDA- 1983/11/01 00:01
PMCR- 1983/11/01
CRDT- 1983/11/01 00:00
PHST- 1983/11/01 00:00 [pubmed]
PHST- 1983/11/01 00:01 [medline]
PHST- 1983/11/01 00:00 [entrez]
PHST- 1983/11/01 00:00 [pmc-release]
AID - 10.1172/JCI111119 [doi]
PST - ppublish
SO  - J Clin Invest. 1983 Nov;72(5):1605-10. doi: 10.1172/JCI111119.