PMID- 6416876
OWN - NLM
STAT- MEDLINE
DCOM- 19840126
LR  - 20190707
IS  - 0014-4827 (Print)
IS  - 0014-4827 (Linking)
VI  - 149
IP  - 2
DP  - 1983 Dec
TI  - Immunohistochemical differences between neurofilaments in perikarya, dendrites 
      and axons. Immunofluorescence study with antisera raised to neurofilament 
      polypeptides (200K, 150K, 70K) isolated by anion exchange chromatography.
PG  - 397-408
AB  - Neurofilament (NF) proteins (70K, 150K and 200K D) were isolated from 2 M urea 
      extracts of bovine spinal cord by anion exchange chromatography. Antisera to the 
      individual NF polypeptides were produced in rabbits and affinity-purified on 
      Sepharose columns prepared with their own antigen. The NF antisera were 
      completely absorbed by their own antigen at protein concentrations that did not 
      decrease the staining when the absorption was conducted with the heterologous NF 
      antigens. Partial absorption (decrease in immunofluorescence titer) occurred at 
      higher concentrations of the heterologous antigens. Cross-reactivity between the 
      polypeptides of the NF triplet could not be detected by double immunodiffusion. 
      The antisera formed immunoprecipitin lines only when reacted with their own 
      antigen. Conversely, cross-reactivity was demonstrated by the immune blotting 
      procedure. Anti-70K stained all three NF polypeptides. Anti-200K and anti-150K 
      stained both 200K and 150K but not 70K, the main reaction being with their own 
      antigen. The antisera were rendered monospecific by adsorption of the common 
      antigenic determinants on Sepharose columns prepared with the heterologous NF 
      antigens. The localization of the NF proteins was studied by immunofluorescence 
      on cryostat sections of rat brain, cerebellum, spinal cord and posterior root 
      ganglia. All NF antisera (anti-70K, anti-150K and anti-200K) stained axons 
      including Purkinje cell baskets with identical pattern. Spinal cord motor 
      neurons, posterior root ganglia neurons and pyramidal neurons in the cerebral 
      cortex stained with anti-70K and anti-200K. No staining of neuronal perikarya and 
      dendrites was observed with anti-150K. Aluminium-induced neurofibrillary tangles 
      in rabbit spinal cord stained with anti-70K and anti-200K. The tangles were not 
      decorated by anti-150K. It is concluded that a marked difference exists in the 
      concentration of 150K depending on the location, i.e., cell body or axon; or, 
      alternatively, that 150K undergoes modification of antigenic sites within the 
      axon so that it may not be recognized immunologically as a component of the 
      neurofilament within perikarya and dendrites.
FAU - Dahl, D
AU  - Dahl D
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Intermediate Filament Proteins)
RN  - 0 (Neurofilament Proteins)
SB  - IM
MH  - Animals
MH  - Axons/*analysis
MH  - Cattle
MH  - Cerebral Cortex/cytology
MH  - Chromatography, Ion Exchange
MH  - Cytoskeleton/*analysis
MH  - Dendrites/*analysis
MH  - Fluorescent Antibody Technique
MH  - Ganglia, Spinal/cytology
MH  - Intermediate Filament Proteins/*analysis/isolation & purification
MH  - Molecular Weight
MH  - Motor Neurons/analysis
MH  - Neurofibrils/analysis
MH  - Neurofilament Proteins
MH  - Neurons/*analysis
MH  - Rabbits
MH  - Rats
MH  - Spinal Cord/cytology
EDAT- 1983/12/01 00:00
MHDA- 1983/12/01 00:01
CRDT- 1983/12/01 00:00
PHST- 1983/12/01 00:00 [pubmed]
PHST- 1983/12/01 00:01 [medline]
PHST- 1983/12/01 00:00 [entrez]
AID - 0014-4827(83)90352-X [pii]
AID - 10.1016/0014-4827(83)90352-x [doi]
PST - ppublish
SO  - Exp Cell Res. 1983 Dec;149(2):397-408. doi: 10.1016/0014-4827(83)90352-x.