PMID- 6424947
OWN - NLM
STAT- MEDLINE
DCOM- 19840530
LR  - 20190720
IS  - 0008-8749 (Print)
IS  - 0008-8749 (Linking)
VI  - 85
IP  - 1
DP  - 1984 Apr 15
TI  - Activation of mouse lymphocytes by anti-immunoglobulin. IV. Stimulation with 
      soluble heterologous anti-delta antibodies.
PG  - 1-14
AB  - Mouse spleen cells were stimulated to proliferate in vitro by soluble 
      affinity-purified heterologous antibodies to mouse delta. Antibodies from goat or 
      rabbit antisera to TEPC 1017, a mouse IgD myeloma protein, were purified on an 
      affinity column of TEPC 1033, a second mouse IgD myeloma protein. Maximum uptake 
      of [3H]thymidine in the range of 60,000 cpm was obtained after 48 hr of culture 
      with anti-delta at concentrations of 50 micrograms/ml. In contrast, the hybridoma 
      10-4.22 anti-delta was nonmitogenic at similar concentrations. The proliferative 
      response was not impaired upon removal of T cells by treatment with an 
      anti-thymocyte serum (ATS), nor by removal of adherent cells by passage of spleen 
      cells over Sephadex G-10 columns and counter-flow centrifugation. Splenic 
      lymphocytes isolated on the fluorescence activated cell sorter (FACS) with 
      intermediate-to-high amounts of surface IgD (sIgD) were responsive to soluble 
      anti-delta, while IgD-negative cells, or cells with low amounts of sIgD, were 
      unresponsive. Spleen cells from mice less than 4 weeks of age, or from mice 
      carrying the X-linked B cell defect (xid), were unresponsive to anti-delta. These 
      results indicate that anti-delta acts similarly to anti-mu in stimulating a 
      proliferative response by later maturing B cells, which are characterized by a 
      high density of sIgD.
FAU - Sieckmann, D G
AU  - Sieckmann DG
FAU - Finkelman, F D
AU  - Finkelman FD
FAU - Thompson, C B
AU  - Thompson CB
FAU - Scher, I
AU  - Scher I
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (Antibodies, Anti-Idiotypic)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Immunoglobulin Fab Fragments)
RN  - 0 (Immunoglobulin Heavy Chains)
RN  - 0 (Immunoglobulin delta-Chains)
RN  - 0 (Immunoglobulin mu-Chains)
SB  - IM
MH  - Animals
MH  - Antibodies, Anti-Idiotypic/*immunology
MH  - Antibodies, Monoclonal/immunology
MH  - B-Lymphocytes/immunology
MH  - Cell Division
MH  - Goats/immunology
MH  - Immunoglobulin Fab Fragments/immunology
MH  - Immunoglobulin Heavy Chains/*immunology
MH  - Immunoglobulin delta-Chains/*immunology
MH  - Immunoglobulin mu-Chains/immunology
MH  - Kinetics
MH  - *Lymphocyte Activation
MH  - Macrophages/immunology
MH  - Male
MH  - Mice
MH  - Rabbits/immunology
MH  - Solubility
MH  - Spleen/cytology
EDAT- 1984/04/15 00:00
MHDA- 1984/04/15 00:01
CRDT- 1984/04/15 00:00
PHST- 1984/04/15 00:00 [pubmed]
PHST- 1984/04/15 00:01 [medline]
PHST- 1984/04/15 00:00 [entrez]
AID - 0008-8749(84)90272-7 [pii]
AID - 10.1016/0008-8749(84)90272-7 [doi]
PST - ppublish
SO  - Cell Immunol. 1984 Apr 15;85(1):1-14. doi: 10.1016/0008-8749(84)90272-7.