PMID- 6435875
OWN - NLM
STAT- MEDLINE
DCOM- 19841218
LR  - 20191023
IS  - 0008-8730 (Print)
IS  - 0008-8730 (Linking)
VI  - 17
IP  - 6
DP  - 1984 Nov
TI  - Two-step cell-death kinetics in vitro during cis-platinum, hydroxyurea and 
      mitomycin incubation.
PG  - 593-600
AB  - A human leukaemic cell line (REH) growing in suspension was incubated with 
      cis-platinum, hydroxyurea and mitomycin C at various concentrations causing 
      complete cell-cycle arrest. At different times the cell suspensions were 
      harvested, diluted 1:1 with a buffer solution, stained without further treatment 
      with a mixture of acridine orange (AO) and ethidium bromide (EB) and analysed 
      with a biparametrical flow cytometer. Fluorescent plastic beads were introduced 
      into the suspensions to provide an internal numerical reference for the control 
      of cell loss. The fluorescence distributions showed three groups of cells: vital 
      cells (V) which were only stained with AO; dead cells in which EB stained 
      cytoplasmic components but not the nuclear DNA (D1), and dead cells which allowed 
      EB to stain both cytoplasm and nuclear DNA (D2). The kinetics of cells entering 
      D1 depended on drug concentration and showed equal characteristics for 
      cis-platinum and mitomycin, but were different for hydroxyurea. The subsequent 
      entry into D2 occurred about 15 hr later and showed no pronounced dependence on 
      drug concentration. Parallel trypan-blue (TB) exclusion tests revealed that TB 
      only stained D2 cells and therefore is not useful for investigating cell-death 
      kinetics during exposure to cell-killing agents.
FAU - Bohmer, R M
AU  - Bohmer RM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Cell Tissue Kinet
JT  - Cell and tissue kinetics
JID - 0174107
RN  - 0 (Antibiotics, Antineoplastic)
RN  - 0 (Mitomycins)
RN  - 50SG953SK6 (Mitomycin)
RN  - Q20Q21Q62J (Cisplatin)
RN  - X6Q56QN5QC (Hydroxyurea)
SB  - IM
MH  - Antibiotics, Antineoplastic/*toxicity
MH  - Cell Line
MH  - Cell Survival/*drug effects
MH  - Cisplatin/*toxicity
MH  - Flow Cytometry
MH  - Humans
MH  - Hydroxyurea/*toxicity
MH  - Kinetics
MH  - Leukemia
MH  - Mitomycin
MH  - Mitomycins/*toxicity
EDAT- 1984/11/01 00:00
MHDA- 1984/11/01 00:01
CRDT- 1984/11/01 00:00
PHST- 1984/11/01 00:00 [pubmed]
PHST- 1984/11/01 00:01 [medline]
PHST- 1984/11/01 00:00 [entrez]
AID - 10.1111/j.1365-2184.1984.tb00618.x [doi]
PST - ppublish
SO  - Cell Tissue Kinet. 1984 Nov;17(6):593-600. doi: 
      10.1111/j.1365-2184.1984.tb00618.x.