PMID- 6438280
OWN - NLM
STAT- MEDLINE
DCOM- 19841227
LR  - 20191101
IS  - 0270-6474 (Print)
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Linking)
VI  - 4
IP  - 11
DP  - 1984 Nov
TI  - Calmodulin stimulates adenylate cyclase activity and increases dopamine 
      activation in bovine retina.
PG  - 2712-7
AB  - Adenylate cyclase activity in bovine retina is highly responsive to Ca2+ and the 
      endogenous Ca2+-binding protein, calmodulin (CaM). CaM stimulated adenylate 
      cyclase activity in washed particulate fractions of bovine retina by 6.6-fold in 
      a Ca2+-dependent manner. Activation of adenylate cyclase activity by CaM was 
      maximal at 0.12 microM free Ca2+. The apparent Ka for calmodulin stimulation of 
      adenylate cyclase was 67 nM and the apparent Vmax was 116 pmol/min/mg of protein 
      above basal activity. Adenylate cyclase activity in bovine retina was stimulated 
      approximately 50% by guanosine 5'-triphosphate (GTP), but the nonhydrolyzable GTP 
      analogue, guanosine-5'-(beta, gamma-imido)triphosphate (Gpp(NH)p), was able to 
      activate the enzyme nearly 5-fold. CaM and Gpp(NH)p appeared to be partially 
      competitive activators of adenylate cyclase in the retina particulate fraction. 
      Dopamine stimulated adenylate cyclase activity in the presence of GTP with an 
      apparent Ka of 1.0 microM and an apparent Vmax of 66 pmol/min/mg of protein. Ca2+ 
      and CaM increased the apparent Vmax of the dopamine-stimulated adenylate cyclase 
      activity more than 2-fold to a level of 146 pmol/min/mg of protein but did not 
      alter the apparent Ka. This suggests that CaM is an endogenous modulator of 
      dopamine-stimulated adenylate cyclase activity in the retina. CaM-stimulated 
      adenylate cyclase activity may be a common component to retina since we found 
      this activity in retinas from rabbit, rat, and goldfish as well as cow.
FAU - Gnegy, M E
AU  - Gnegy ME
FAU - Muirhead, N
AU  - Muirhead N
FAU - Roberts-Lewis, J M
AU  - Roberts-Lewis JM
FAU - Treisman, G
AU  - Treisman G
LA  - eng
GR  - R01 MH36044-01/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Calmodulin)
RN  - 34273-04-6 (Guanylyl Imidodiphosphate)
RN  - 526U7A2651 (Egtazic Acid)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - EC 4.6.1.1 (Adenylyl Cyclases)
RN  - SY7Q814VUP (Calcium)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Adenylyl Cyclases/*metabolism
MH  - Animals
MH  - Calcium/pharmacology
MH  - Calmodulin/*pharmacology
MH  - Cattle
MH  - Dopamine/*pharmacology
MH  - Egtazic Acid/pharmacology
MH  - Enzyme Activation/drug effects
MH  - Guanosine Triphosphate/pharmacology
MH  - Guanylyl Imidodiphosphate/pharmacology
MH  - Retina/*enzymology
PMC - PMC6564732
EDAT- 1984/11/01 00:00
MHDA- 1984/11/01 00:01
PMCR- 1985/05/01
CRDT- 1984/11/01 00:00
PHST- 1984/11/01 00:00 [pubmed]
PHST- 1984/11/01 00:01 [medline]
PHST- 1984/11/01 00:00 [entrez]
PHST- 1985/05/01 00:00 [pmc-release]
AID - 10.1523/JNEUROSCI.04-11-02712.1984 [doi]
PST - ppublish
SO  - J Neurosci. 1984 Nov;4(11):2712-7. doi: 10.1523/JNEUROSCI.04-11-02712.1984.